Horseradish peroxidase inactivation: heme destruction and influence of polyethylene glycol.
Horseradish peroxidase inactivation: heme destruction and influence of polyethylene glycol.
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辣根过氧化物酶失活：血红素破坏和聚乙二醇的影响
DOI：
10.1038/srep03126
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发表时间：
2013-11-04
影响因子：
4.6
通讯作者：
Lu, Junhe
中科院分区：
文献类型：
--
作者：
Mao, Liang;Luo, Siqiang;Huang, Qingguo;Lu, Junhe
Horseradish peroxidase (HRP) mediates efficient conversion of many phenolic contaminants and thus has potential applications for pollution control. Such potentially important applications suffer however from the fact that the enzyme becomes quickly inactivated during phenol oxidation and polymerization. The work here provides the first experimental data of heme consumption and iron releases to support the hypothesis that HRP is inactivated by heme destruction. Product of heme destruction is identified using liquid chromatography with mass spectrometry. The heme macrocycle destruction involving deprivation of the heme iron and oxidation of the 4-vinyl group in heme occurs as a result of the reaction. We also demonstrated that heme consumption and iron releases resulting from HRP destruction are largely reduced in the presence of polyethylene glycol (PEG), providing the first evidence to indicate that heme destruction is effectively suppressed by co-dissolved PEG. These findings advance a better understanding of the mechanisms of HRP inactivation.
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影响因子：
4.1
作者：
GEORGE, P
通讯作者：
GEORGE, P
影响因子：
11.4
作者：
Huang, QG;Weber, WJ
通讯作者：
Weber, WJ
影响因子：
3.9
作者：
Junker, Katja;Zandomeneghi, Giorgia;Walde, Peter
通讯作者：
Walde, Peter
影响因子：
56.9
作者：
KLIBANOV, AM;TU, TM;SCOTT, KP
通讯作者：
SCOTT, KP
影响因子：
11.4
作者：
Mao, Liang;Lu, Junhe;Huang, Qingguo
通讯作者：
Huang, Qingguo