Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopy

Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopy
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傅里叶变换红外光谱研究极低频磁场对HeLa活细胞膜蛋白结构的影响

DOI:
10.1002/bem.10120
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发表时间:
2003
影响因子:
1.9
通讯作者:
K. Aizawa
K. Aizawa
中科院分区:
生物学4区
文献类型:
--
作者:
T. Ikehara;H. Yamaguchi;K. Hosokawa;H. Miyamoto;K. Aizawa

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用衰减全反射红外光谱技术研究了50赫兹磁场(最大41.7~43.6mT)对活体HeLa细胞膜蛋白结构的影响。暴露1min,使酰胺I带的峰值吸光度移动到一个较小的波数,降低了酰胺II带的峰值吸光度,增加了1600 cm−1附近的吸光度。这些结果表明,超低频磁场对多肽键的N-H面内弯曲和C-N伸展振动有可逆的影响,并改变了细胞膜蛋白中α-螺旋和β-折叠的二级结构。《生物电磁学》24:457-464,2003。©2003 Wiley-Liss公司
The effects of exposure to a 50 Hz magnetic field (maximum of 41.7 to 43.6 mT) on the membrane protein structures of living HeLa cells were studied using attenuated total reflection infrared spectroscopy. One min of such exposure shifted peak absorbance of the amide I band to a smaller wave number, reduced peak absorbance of the amide II band, and increased absorbance at around 1600 cm−1. These results suggest that exposure to the ELF magnetic field has reversible effects on the N–H inplane bending and C–N stretching vibrations of peptide linkages, and changes the secondary structures of α‐helix and β‐sheet in cell membrane proteins. Bioelectromagnetics 24:457–464, 2003. © 2003 Wiley‐Liss, Inc.
DOI: 10.1002/bem.2250120307
发表时间: 1991
影响因子: 1.9
作者:
Smith,OM;Goodman,EM;Greenebaum,B;Tipnis,P
通讯作者: Tipnis,P
DOI: --
发表时间: 1987
期刊: The Journal of biological chemistry
影响因子: --
作者:
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通讯作者: Martonosi,A
一种通过傅里叶变换红外光谱原位监测哺乳动物细胞生长和抑制的技术。
DOI: 10.1016/0003-2697(88)90040-1
发表时间: 1988
影响因子: 2.9
作者:
Hutson,TB;Mitchell,ML;Keller,JT;Long,DJ;Chang,MJ
通讯作者: Chang,MJ