The ultrastructural organization of endoplasmic reticulum-plasma membrane contacts is conserved in epithelial cells.
The ultrastructural organization of endoplasmic reticulum-plasma membrane contacts is conserved in epithelial cells.
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DOI：
10.1091/mbc.e21-11-0534-t
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发表时间：
2022-10-01
影响因子：
3.3
通讯作者：
Stefan, Christopher J.
中科院分区：
文献类型：
--
作者：
Chung, Gary Hong Chun;Lorvellec, Maelle;Gissen, Paul;Pichaud, Franck;Burden, Jemima J.;Stefan, Christopher J.
内质网和质膜之间的接触（ER-PM接触）在膜脂和钙动力学中具有重要作用，但它们在极化上皮细胞中的组织尚未得到彻底描述。在这里，我们使用电子显微镜检查小鼠肝脏肝细胞中的 ER-PM 接触，首次对哺乳动物上皮组织中的 ER-PM 接触进行全面的超微结构研究。我们的定量分析揭示了与顶端、侧向和基底 PM 域空间相关的显着不同的 ER-PM 接触结构。值得注意的是，我们发现在形成肝细胞之间细胞间连接的侧 PM 域存在广泛的 ER-PM 接触网络。此外，ER-PM接触的空间组织在上皮球体中是保守的，这表明ER-PM接触可能在上皮细胞结构中发挥保守的作用。与这一观点一致，我们发现 ER-PM 接触处的 ORP5 活性调节 HepG2 细胞的顶端-基底外侧纵横比。因此，ER-PM 接触在跨上皮细胞类型的 PM 结构域结构中具有保守的分布和关键作用。
Contacts between the endoplasmic reticulum and the plasma membrane (ER-PM contacts) have important roles in membrane lipid and calcium dynamics, yet their organization in polarized epithelial cells has not been thoroughly described. Here we examine ER-PM contacts in hepatocytes in mouse liver using electron microscopy, providing the first comprehensive ultrastructural study of ER-PM contacts in a mammalian epithelial tissue. Our quantitative analyses reveal strikingly distinct ER-PM contact architectures spatially linked to apical, lateral, and basal PM domains. Notably, we find that an extensive network of ER-PM contacts exists at lateral PM domains that form intercellular junctions between hepatocytes. Moreover, the spatial organization of ER-PM contacts is conserved in epithelial spheroids, suggesting that ER-PM contacts may serve conserved roles in epithelial cell architecture. Consistent with this notion, we show that ORP5 activity at ER-PM contacts modulates the apical-basolateral aspect ratio in HepG2 cells. Thus ER-PM contacts have a conserved distribution and crucial roles in PM domain architecture across epithelial cell types.
调节磷脂酰肌醇4,5-双磷酸的连接缝连接通信。
DOI：
10.1083/jcb.200610144
发表时间：
2007-06-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
van Zeijl L;Ponsioen B;Giepmans BN;Ariaens A;Postma FR;Várnai P;Balla T;Divecha N;Jalink K;Moolenaar WH
通讯作者：
Moolenaar WH