Neofunctionalization of a duplicate hatching enzyme gene during the evolution of teleost fishes.
Neofunctionalization of a duplicate hatching enzyme gene during the evolution of teleost fishes.
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DOI：
10.1186/s12862-014-0221-0
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发表时间：
2014-10-19
影响因子：
3.4
通讯作者：
Yasumasu S
中科院分区：
文献类型：
--
作者：
Sano K;Kawaguchi M;Watanabe S;Yasumasu S
真骨鱼孵化酶基因的复制和随后的neofunctionalization发生在真骨鱼和耳头鱼的共同祖先，产生两个属于不同系统发育分支（分支I和II）的基因。在真骨鱼类中，进化枝I酶继承了祖先酶的活性，该酶通过切割卵被膜蛋白的N-末端区域来使卵被膜膨胀。进化枝II酶获得了两个特异性切割位点，N-ZPd和mid-ZPd，但失去了祖先活性。因此，euteleostean进化枝II酶承担了一个新的功能，溶解的鸡蛋信封的合作行动与进化枝I酶。然而，在耳头类中，进化枝II基因在进化过程中丢失。因此，在耳头类的晚期类群中，只有进化枝I酶存在以使卵被膜膨胀。我们评估了卵包膜消化特性的进化枝I和II酶在Gonorynchiformes，早期分歧组的耳头目，使用遮流鱼，并比较他们的消化与其他鱼类。最后，我们提出了一个新功能化过程的假设。遮流鱼进化枝II酶裂解N-ZPd，但不中间ZPd，并没有引起溶解的卵包膜。我们的结论是，新功能是不完整的耳首分支II酶。进化枝I和进化枝II酶特性的比较意味着进化枝II酶的特异性在重复事件后的进化过程中逐渐改变，并且需要底物的变化来添加中间ZPd位点和N-末端区域的活性损失。我们推断的过程中的新功能化的分支II酶的基因复制后。祖先进化枝II基因在真骨鱼纲和耳头鱼纲的共同祖先谱系中获得了N-ZPd切割活性。随后，收购的切割活性在中间ZPd网站和N-末端区域的切割活性的损失发生在进化的真骨鱼类，但不是耳头类。进化枝II酶提供了一个新功能基因的发展的例子，对于该新功能基因，底物卵包膜蛋白已经适应了相应酶的特异性的逐渐变化。
Duplication and subsequent neofunctionalization of the teleostean hatching enzyme gene occurred in the common ancestor of Euteleostei and Otocephala, producing two genes belonging to different phylogenetic clades (clade I and II). In euteleosts, the clade I enzyme inherited the activity of the ancestral enzyme of swelling the egg envelope by cleavage of the N-terminal region of egg envelope proteins. The clade II enzyme gained two specific cleavage sites, N-ZPd and mid-ZPd but lost the ancestral activity. Thus, euteleostean clade II enzymes assumed a new function; solubilization of the egg envelope by the cooperative action with clade I enzyme. However, in Otocephala, the clade II gene was lost during evolution. Consequently, in a late group of Otocephala, only the clade I enzyme is present to swell the egg envelope. We evaluated the egg envelope digestion properties of clade I and II enzymes in Gonorynchiformes, an early diverging group of Otocephala, using milkfish, and compared their digestion with those of other fishes. Finally, we propose a hypothesis of the neofunctionalization process. The milkfish clade II enzyme cleaved N-ZPd but not mid-ZPd, and did not cause solubilization of the egg envelope. We conclude that neofunctionalization is incomplete in the otocephalan clade II enzymes. Comparison of clade I and clade II enzyme characteristics implies that the specificity of the clade II enzymes gradually changed during evolution after the duplication event, and that a change in substrate was required for the addition of the mid-ZPd site and loss of activity at the N-terminal region. We infer the process of neofunctionalization of the clade II enzyme after duplication of the gene. The ancestral clade II gene gained N-ZPd cleavage activity in the common ancestral lineage of the Euteleostei and Otocephala. Subsequently, acquisition of cleavage activity at the mid-ZPd site and loss of cleavage activity in the N-terminal region occurred during the evolution of Euteleostei, but not of Otocephala. The clade II enzyme provides an example of the development of a neofunctional gene for which the substrate, the egg envelope protein, has adapted to a gradual change in the specificity of the corresponding enzyme.
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影响因子：
3.4
作者：
Kawaguchi M;Hiroi J;Miya M;Nishida M;Iuchi I;Yasumasu S
通讯作者：
Yasumasu S
DOI：
10.1073/pnas.1632368100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Inoue, K;Naruse, K;Takei, Y
通讯作者：
Takei, Y
影响因子：
21.3
作者：
Jovine, L;Qi, HY;Wassarman, PM
通讯作者：
Wassarman, PM
影响因子：
5.4
作者：
Kawaguchi, Mari;Yasumasu, Shigeki;Nishida, Mutsumi
通讯作者：
Nishida, Mutsumi
影响因子：
5.4
作者：
Sano, Kaori;Kawaguchi, Mari;Yasumasu, Shigeki
通讯作者：
Yasumasu, Shigeki