A non-invasive restraining system for awake mouse imaging.
A non-invasive restraining system for awake mouse imaging.
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DOI：
10.1016/j.jneumeth.2017.06.008
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发表时间：
2017-08-01
影响因子：
3
通讯作者：
Rajah MN
中科院分区：
文献类型：
--
作者：
Madularu D;Mathieu AP;Kumaragamage C;Reynolds LM;Near J;Flores C;Rajah MN
临床前神经成像允许在实验室动物（例如小鼠和大鼠）中评估大脑解剖结构、连接性和功能。大多数这些研究都是在麻醉下进行的，以避免在扫描过程中移动。由于与麻醉成像相关的局限性，最近已努力在清醒动物中进行啮齿动物成像研究，这些动物习惯于在这些情况下使用的约束系统。到目前为止，只有一个这样的系统可用于小鼠扫描（Animal Imaging Research，Boston，MA，USA），其将射频线圈电子器件与约束元件集成在一起，这种方法虽然在清醒成像期间有效地减少头部运动，但具有一些局限性。在目前的报告中，我们提出了一种新的鼠标约束系统，解决了这些限制。在33只清醒小鼠中，通过两次连续功能性MRI扫描（共20分钟）的三维线性头部运动来评价约束系统的有效性。头部的运动是最小的，记录在大约12%的时间序列。驯化过程中呼吸速率下降，而推注计数保持不变。功能采集期间的身体运动对磁场（B0）均匀性没有显著影响。与市售系统相比，当前设计的受益是双重的：1）它与一系列市售弹簧圈兼容，2）它允许神经成像与涉及颅内插管的其他已建立技术（即微输注和光遗传学）配对。
Preclinical neuroimaging allows for the assessment of brain anatomy, connectivity and function in laboratory animals, such as mice and rats. Most of these studies are performed under anesthesia to avoid movement during the scanning sessions. Due to the limitations associated with anesthetized imaging, recent efforts have been made to conduct rodent imaging studies in awake animals, habituated to the restraint systems used in these instances. As of now, only one such system is commercially available for mouse scanning (Animal Imaging Research, Boston, MA, USA) integrating the radiofrequency coil electronics with the restraining element, an approach which, although effective in reducing head motion during awake imaging, has some limitations. In the current report, we present a novel mouse restraining system that addresses some of these limitations. The effectiveness of the restraining system was evaluated in terms of three-dimensional linear head movement across two consecutive functional MRI scans (total 20 min) in 33 awake mice. Head movement was minimal, recorded in roughly 12% of the time-series. Respiration rate during the acclimation procedure dropped while the bolus count remained unchanged. Body movement during functional acquisitions did not have a significant effect on magnetic field (B0) homogeneity. Compared to the commercially available system, the benefit of the current design is two-fold: 1) it is compatible with a range of commercially-available coils, and 2) it allows for the pairing of neuroimaging with other established techniques involving intracranial cannulation (i.e. microinfusion and optogenetics).
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影响因子：
5.7
作者：
Jenkinson, M;Bannister, P;Smith, S
通讯作者：
Smith, S
影响因子：
6.8
作者：
Kenkel WM;Yee JR;Moore K;Madularu D;Kulkarni P;Gamber K;Nedelman M;Ferris CF
通讯作者：
Ferris CF
影响因子：
5.7
作者：
Liang Z;Watson GD;Alloway KD;Lee G;Neuberger T;Zhang N
通讯作者：
Zhang N
DOI：
10.1126/science.aac9698
发表时间：
2016-01-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ferenczi EA;Zalocusky KA;Liston C;Grosenick L;Warden MR;Amatya D;Katovich K;Mehta H;Patenaude B;Ramakrishnan C;Kalanithi P;Etkin A;Knutson B;Glover GH;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
3.8
作者：
Baldwin, Lesley N.;Wachowicz, Keith;Fallone, B. Gino
通讯作者：
Fallone, B. Gino