Polycomb proteins remain bound to chromatin and DNA during DNA replication in vitro.
Polycomb proteins remain bound to chromatin and DNA during DNA replication in vitro.
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DOI：
10.1016/j.cell.2009.02.017
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发表时间：
2009-04-03
期刊：
影响因子：
64.5
通讯作者：
Butler JD
中科院分区：
文献类型：
--
作者：
Francis NJ;Follmer NE;Simon MD;Aghia G;Butler JD
基因的转录状态可以通过发育期间的多轮细胞分裂来维持。这种表观遗传效应被认为反映了染色质折叠和组蛋白修饰或靶基因变体的可遗传变化，但对这些染色质特征如何通过细胞分裂遗传知之甚少。维持转录状态的一个特别挑战是DNA复制，它破坏或稀释染色质相关蛋白和组蛋白修饰。PRC1类Polycomb Group蛋白复合物对于发育是必不可少的，并且被认为通过改变染色质折叠和组蛋白修饰来遗传性地沉默转录。目前尚不清楚这些复合物及其作用是否在DNA复制期间维持或随后重新建立。我们发现，当PRC1类Polycomb复合物结合的染色质或DNA在体外复制，Polycomb复合物保持绑定到复制的模板。通过DNA复制保留Polycomb蛋白可能有助于通过细胞分裂维持转录沉默。
The transcriptional status of a gene can be maintained through multiple rounds of cell division during development. This epigenetic effect is believed to reflect heritable changes in chromatin folding and histone modifications or variants at target genes, but little is known about how these chromatin features are inherited through cell division. A particular challenge for maintaining transcription states is DNA replication, which disrupts or dilutes chromatin associated proteins and histone modifications. PRC1-class Polycomb Group protein complexes are essential for development, and are thought to heritably silence transcription by altering chromatin folding and histone modifications. It is not known whether these complexes and their effects are maintained during DNA replication or subsequently re-established. We find that when PRC1-class Polycomb complex-bound chromatin or DNA is replicated in vitro, Polycomb complexes remain bound to replicated templates. Retention of Polycomb proteins through DNA replication may contribute to maintenance of transcriptional silencing through cell division.
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