Bioprospecting of wild type ethanologenic yeast for ethanol fuel production from wastewater-grown microalgae.
Bioprospecting of wild type ethanologenic yeast for ethanol fuel production from wastewater-grown microalgae.
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DOI：
10.1186/s13068-021-01925-x
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发表时间：
2021-04-09
影响因子：
6.3
通讯作者：
Orta Ledesma MT
中科院分区：
文献类型：
--
作者：
Romero-Frasca E;Velasquez-Orta SB;Escobar-Sánchez V;Tinoco-Valencia R;Orta Ledesma MT
野生型酵母已成功用于获取食品，但其作为大规模乙醇燃料生产的发酵微生物的全部潜力仍有待确定。在这项研究中，评估了从二次流出物中分离出的野生型产乙醇酵母发酵糖化微藻糖的能力。通过对核糖体簇的内部转录间隔区 1 和 2 区域进行测序，鉴定了废水中的酵母菌种。同时，微藻生物质糖通过酸水解被糖化，产生 5.0±0.3 g L−1 的可发酵糖。在不同的初始糖浓度和发酵时间下测试了水解微藻液中酵母的葡萄糖消耗和乙醇产量。主要产乙醇酵母菌种被鉴定为念珠菌属 (Candida sp.)，该菌株和酿酒酵母的葡萄糖消耗在最佳条件下分别达到初始浓度的 75% 和 87%。两个物种的乙醇产量相对相似，达到每克葡萄糖 0.45±0.05 克乙醇（酿酒酵母）和每克葡萄糖 0.46±0.05 克乙醇（念珠菌属）。总体而言，这些结果提供了对特定野生型念珠菌物种发酵能力的初步了解，以及它们在寻求提高成本效益的乙醇工业中的潜在作用。 在线版本包含可在 10.1186/s13068-021-01925-x 获取的补充材料。
Wild-type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined. In this study, wild-type ethanologenic yeasts isolated from a secondary effluent were assessed for their capability to ferment saccharified microalgae sugars. Yeast species in wastewater were identified sequencing the Internal Transcribed Spacers 1 and 2 regions of the ribosomal cluster. Concurrently, microalgae biomass sugars were saccharified via acid hydrolysis, producing 5.0 ± 0.3 g L−1 of fermentable sugars. Glucose consumption and ethanol production of yeasts in hydrolyzed-microalgae liquor were tested at different initial sugar concentrations and fermentation time. The predominant ethanologenic yeast species was identified as Candida sp., and glucose consumption for this strain and S. cerevisiae achieved 75% and 87% of the initial concentration at optimal conditions, respectively. Relatively similar ethanol yields were determined for both species, achieving 0.45 ± 0.05 (S. cerevisiae) and 0.46 ± 0.05 g ethanol per g glucose (Candida sp.). Overall, the results provide a first insight of the fermentation capacities of specific wild-type Candida species, and their potential role in ethanol industries seeking to improve their cost-efficiency. The online version contains supplementary material available at 10.1186/s13068-021-01925-x.
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影响因子：
5.7
作者：
Behera S;Singh R;Arora R;Sharma NK;Shukla M;Kumar S
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