Stem cell-seeded 3D-printed scaffolds combined with self-assembling peptides for bone defect repair.
Stem cell-seeded 3D-printed scaffolds combined with self-assembling peptides for bone defect repair.
复制标题
干细胞接种的 3D 打印支架与自组装肽相结合，用于骨缺损修复。
作者：
Haixia Xu;Chengqiang Wang;Chun Liu;Jianjun Li;Ziyue Peng;Jiasong Guo;Lixin Zhu
感染、肿瘤、创伤等引起的骨缺损临床上仍难以治疗。骨组织工程（BTE）在促进骨缺损修复方面具有巨大的应用前景。聚己内酯 (PCL) 是制造 BTE 支架的常用材料。此外，自组装肽（SAP）可以充当细胞外基质并促进成骨和血管生成。在这项工作中，通过3D打印构建了PCL支架，然后与骨髓间充质干细胞（BMSC）和SAP集成。该研究旨在评估PCL/BMSC/SAP种植体的骨修复能力。通过 Cell Counting Kit-8 评估 PCL/SAP 支架中的 BMSC 增殖。通过碱性磷酸酶染色和活性测定评估 PCL/SAP 支架中培养的 BMSC 的体外成骨作用。还进行了酶联免疫吸附测定来检测成骨因子的水平。通过划痕、Transwell 和成管实验评估来自 3D 培养系统的 BMSC 条件培养基对人脐静脉内皮细胞 (HUVEC) 迁移和血管生成的影响。体内移植8周后，使用放射线照相和组织学评估骨再生，并使用免疫组织化学染色检测新生血管形成。体外结果表明，与PCL支架相比，PCL/SAP支架促进BMSC增殖和成骨，并且与PCL/BMSC CM相比，PCL/BMSC/SAP条件培养基（CM）增强HUVEC迁移和血管生成。体内结果显示，与空白对照组、PCL组和PCL/BMSC组相比，PCL/BMSC/SAP组骨和血管形成显着增加。因此，将 BMSC 接种的 3D 打印 PCL 和 SAP 结合起来可以成为治疗骨缺损的有效方法。
Bone defects caused by infection, tumor, trauma and so on remain difficult to treat clinically. Bone tissue engineering (BTE) has great application prospect in promoting bone defect repair. Polycaprolactone (PCL) is a commonly used material for creating BTE scaffolds. In addition, self-assembling peptides (SAPs) can function as the extracellular matrix and promote osteogenesis and angiogenesis. In the work, a PCL scaffold was constructed by 3D printing, then integrated with bone marrow mesenchymal stem cells (BMSCs) and SAPs. The research aimed to assess the bone repair ability of PCL/BMSC/SAP implants. BMSC proliferation in PCL/SAP scaffolds was assessed via Cell Counting Kit-8. In vitro osteogenesis of BMSCs cultured in PCL/SAP scaffolds was assessed by alkaline phosphatase staining and activity assays. Enzyme linked immunosorbent assays were also performed to detect the levels of osteogenic factors. The effects of BMSC-conditioned medium from 3D culture systems on the migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) were assessed by scratch, transwell, and tube formation assays. After 8 weeks of in vivo transplantation, radiography and histology were used to evaluate bone regeneration, and immunohistochemistry staining was utilized to detect neovascularization. In vitro results demonstrated that PCL/SAP scaffolds promoted BMSC proliferation and osteogenesis compared to PCL scaffolds, and the PCL/BMSC/SAP conditional medium (CM) enhanced HUVEC migration and angiogenesis compared to the PCL/BMSC CM. In vivo results showed that, compared to the blank control, PCL, and PCL/BMSC groups, the PCL/BMSC/SAP group had significantly increased bone and blood vessel formation. Thus, the combination of BMSC-seeded 3D-printed PCL and SAPs can be an effective approach for treating bone defects.
DOI：
10.1146/annurev-bioeng-062117-121139
发表时间：
2018-06-04
影响因子：
9.7
作者：
Morgan EF;Unnikrisnan GU;Hussein AI
通讯作者：
Hussein AI