miRNeye: a microRNA expression atlas of the mouse eye.
miRNeye: a microRNA expression atlas of the mouse eye.
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DOI：
10.1186/1471-2164-11-715
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发表时间：
2010-12-20
期刊：
影响因子：
4.4
通讯作者：
Banfi S
中科院分区：
文献类型：
--
作者：
Karali M;Peluso I;Gennarino VA;Bilio M;Verde R;Lago G;Dollé P;Banfi S
microRNA（miRNAs）是生物过程的关键调节因子。为了确定眼睛中的miRNA功能，必须确定其空间和时间分布的高分辨率谱。在这份报告中，我们提出了第一个全面调查的眼睛组织中的miRNA表达，使用微阵列和RNA原位杂交（ISH）程序。我们首先通过基因芯片检测了成年小鼠视网膜、透镜、角膜和视网膜色素上皮中miRNAs的表达谱。每种组织都表现出明显不同的miRNA富集模式，聚类分析鉴定了在特定眼组织或其组合中表现出主要表达的miRNA组。接下来，我们对超过220种miRNAs进行了RNA ISH，包括那些通过微阵列显示最高表达水平的miRNAs，并生成了发育和成年野生型小鼠眼中miRNAs的高分辨率表达图谱，该图谱可以以公开的网络数据库的形式访问。我们发现，122个miRNAs在不同发育阶段的眼睛中显示出限制性表达结构域，其中大多数在神经视网膜的一个或多个细胞层中表达。该分析揭示了在眼组织中具有差异表达的miRNA，并提供了在小鼠眼发育期间其组织特异性分布的详细图谱。这两种方法的结合提供了一个有价值的资源，以破译特定的miRNA和miRNA簇的不同眼结构的发展的贡献。
MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures.
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影响因子：
64.8
作者：
Cordes KR;Sheehy NT;White MP;Berry EC;Morton SU;Muth AN;Lee TH;Miano JM;Ivey KN;Srivastava D
通讯作者：
Srivastava D
影响因子：
2.5
作者：
Li, Yan;Piatigorsky, Joram
通讯作者：
Piatigorsky, Joram
DOI：
10.1073/pnas.0914785107
发表时间：
2010-08-31
影响因子：
11.1
作者：
Conte, Ivan;Carrella, Sabrina;Banfi, Sandro
通讯作者：
Banfi, Sandro
影响因子：
4.4
作者：
Karali, Marianthi;Peluso, Ivana;Banfi, Sandro
通讯作者：
Banfi, Sandro
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S