Molecular and Catalytic Properties of Glutathione Peroxidase Family Proteins from Pinus tabulaeformis
Molecular and Catalytic Properties of Glutathione Peroxidase Family Proteins from Pinus tabulaeformis
复制标题
油松谷胱甘肽过氧化物酶家族蛋白的分子和催化特性
DOI：
10.1007/s11105-013-0692-y
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发表时间：
2014-07
影响因子：
2.1
通讯作者：
Yang, Hai-Ling
中科院分区：
文献类型：
--
作者：
Zhao, Li;Han, Xue-Min;Wang, Wei;Yang, Hai-Ling
谷胱甘肽过氧化物酶（GPX）是保护细胞免受活性氧引起的氧化损伤的关键酶之一。先前对植物GPX的研究主要集中在被子植物上。相比之下，关于其分子的信息却很少。
Glutathione peroxidase (GPX) is one of the key enzymes that protect cells against oxidative damage caused by reactive oxygen species. Previous studies of plant GPXs focused mainly on angiosperms. In contrast, little information is available on the molecular characteristics of this gene family in gymnosperms. In this study, four GPX genes (PtaGPX1, 2, 3, and 4) were cloned from the gymnosperm Pinus tabulaeformis, which showed high protein sequence identity and similar expression patterns in various tissues. The four Pinus GPX proteins were expressed in Escherichia coli, and the purified proteins used thioredoxin, but not glutathione, as an electron donor. The four Pinus GPXs showed different enzymatic activities and kinetic characteristics, suggesting functional divergence. Two conserved Cys residues (corresponding to Cys44 and Cys92 of PtaGPX3) were identified in all plant GPXs, and their functions were assessed using site-directed mutagenesis. Cys44 and Cys92 of PtaGPX3 could form an intramolecular disulfide bond under oxidizing conditions. These two residues were critical components of active sites and contributed to catalytic activity. This study provides novel insights into the functional divergence and catalytic properties of the GPX family in gymnosperms.
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影响因子：
5.6
作者：
Koh, Cha San;Didierjean, Claude;Corbier, Catherine
通讯作者：
Corbier, Catherine
影响因子：
3.5
作者：
Agrawal, GK;Rakwal, R;Agrawal, VP
通讯作者：
Agrawal, VP
水稻 (Oryza sativa) tau 类谷胱甘肽转移酶的生化和生理学特征
影响因子：
6.5
作者：
Yang, Xue;Sun, Wu;Zeng, Qing-Yin
通讯作者：
Zeng, Qing-Yin
DOI：
10.1046/j.1432-1033.2002.02905.x
发表时间：
2002-05-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Herbette, S;Lenne, C;Roeckel-Drevet, P
通讯作者：
Roeckel-Drevet, P
影响因子：
5.4
作者：
Iqbal, Aqib;Yabuta, Yukinori;Shigeoka, Shigeru
通讯作者：
Shigeoka, Shigeru