Clonal Characteristics of T-Cell Receptor Repertoires in Violent and Non-violent Patients With Schizophrenia.
Clonal Characteristics of T-Cell Receptor Repertoires in Violent and Non-violent Patients With Schizophrenia.
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暴力和非暴力精神分裂症患者 T 细胞受体库的克隆特征
DOI：
10.3389/fpsyt.2018.00403
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发表时间：
2018
影响因子：
4.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Li Q;Zhou J;Cao X;Liu Q;Li Q;Li W;Wang X
背景：炎症和变性过程中T细胞功能的激活或受损可导致精神分裂症的发病风险和进展。本研究采用免疫谱系测序法，对暴力型和非暴力型精神分裂症患者外周血中T细胞受体β可变链(TRBV)的分布进行了研究。方法：选择10例暴力型和10例非暴力型精神分裂症患者和8例健康对照。采用简明精神病学评定量表(BPRS)评定患者的精神症状。采用改良的外显攻击量表(MOAS)评定攻击水平。用多重聚合酶链式反应和高通量测序方法检测TRBV的互补决定区3(CDR3)。结果：三组TCR曲谱多样性在Shannon-Wiener多样性指数或逆Simpson多样性指数上无显著差异。对TRBV组成和丰度的主成分分析表明，主成分1和主成分2可以解释总变异的28.88%和13.24%。精神分裂症患者(暴力性和非暴力性)的V基因分布与健康对照组有显著差异。特别是，暴力性精神分裂症组TRBV2的出现频率显著高于非暴力性精神分裂症组和健康对照组，TRBV7-2的出现频率在非暴力性精神分裂症组显著高于暴力性精神分裂症组和健康对照组。结论：暴力型和非暴力型精神分裂症患者均携带异常的T细胞受体，为探讨精神分裂症暴力行为的病因提供了有用的线索。
Background: Activated or impaired T-cell function in inflammatory and degenerative process can contribute to the risk and progression of schizophrenia. This study used immune repertoire sequencing to investigate the T-cell receptor beta variable chain (TRBV) presence in blood mononuclear cells in the violent or non-violent schizophrenic patients. Methods: Ten violent and 10 non-violent schizophrenic patients and 8 matched healthy controls were enrolled. The Brief Psychiatric Rating Scale (BPRS) was used to evaluate patients' psychiatric symptoms. The level of aggression was assessed using the Modified Overt Aggression Scale (MOAS). The complementarity-determining region 3 (CDR3) of TRBV was detected using multiplex-PCR and high-throughput sequencing. Results: The TCR repertoire diversity were no significant differences in the Shannon–Wiener or inverse Simpson diversity index between three groups. Principal component analysis (PCA) of TRBV composition and abundance showed that principal component 1 and principal component 2 can explain 28.88 and 13.24% of total variation, respectively. Schizophrenic patients (violent and non-violent) had significantly different V gene distribution compared to healthy controls. In particular, TRBV2 occurred at a significantly higher frequency in the violent schizophrenia group than in the non-violent schizophrenia and healthy control groups, and TRBV7-2 occurred at a significantly higher frequency in the non-violent schizophrenia group than in the violent schizophrenia and healthy control groups. Conclusions: The results suggest that violent and non-violent schizophrenic patients carry abnormal T-cell receptor repertoires, and these data provide a useful clue to explore the etiology of violent behavior in schizophrenia.
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影响因子：
64.8
作者：
Filiano AJ;Xu Y;Tustison NJ;Marsh RL;Baker W;Smirnov I;Overall CC;Gadani SP;Turner SD;Weng Z;Peerzade SN;Chen H;Lee KS;Scott MM;Beenhakker MP;Litvak V;Kipnis J
通讯作者：
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作者：
Derecki, Noel C.;Cardani, Amber N.;Yang, Chun Hui;Quinnies, Kayla M.;Crihfield, Anastasia;Lynch, Kevin R.;Kipnis, Jonathan
通讯作者：
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影响因子：
9.3
作者：
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影响因子：
17.7
作者：
Keers, Robert;Ullrich, Simone;Coid, Jeremy W.
通讯作者：
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