In vivo determination of genotoxicity induced by metals from orthodontic appliances using micronucleus and comet assays.
In vivo determination of genotoxicity induced by metals from orthodontic appliances using micronucleus and comet assays.
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使用微核和彗星测定体内测定正畸矫治器中的金属诱导的遗传毒性。
DOI：
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发表时间：
2008
影响因子：
0.4
通讯作者：
R. Medina
中科院分区：
文献类型：
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作者：
G. H. Westphalen;L. Menezes;D. Prá;G. G. Garcia;V. M. Schmitt;J. Henriques;R. Medina
正畸器械通常由不锈钢制成，其中含有与DNA损伤有关的镍、铬和铁等金属。本研究的目的是利用口腔细胞微核(MN)和彗星(CA)试验，对20名健康患者(16+/-2.5岁)进行正畸治疗(固定矫治器-基本成分：不锈钢合金)，以确定与正畸固定矫治器相关的遗传毒性。CA评估的初级DNA损伤水平在正畸矫治器放置前(1.5+/-1.05损伤指数-DI)或放置正畸矫治器后10天(2.5+/-3.08DI)较低，并且在这两个时间点之间没有显著变化(p=0.0913)。相反，在治疗开始后30天，微核率显著增加(p=0.0236)。在本研究中，MN法比CA法更敏感。为了评估正畸固定矫治器的遗传毒性潜力，有必要进行其他研究，这与关于正畸患者的这些影响的长期研究有关。
Orthodontic appliances are usually made of stainless steel, which contains metals such as nickel, chromium and iron that have been associated with DNA damage. The aim of the present study was to determine the genetic toxicity associated with orthodontic fixed appliances in twenty healthy patients (16 +/- 2.5 years) undergoing orthodontic treatment (fixed appliances - basic composition: stainless steel alloy), using the micronucleus (MN) and comet (CA) assays in buccal cells. Primary DNA damage level, as assessed by the CA, was low either before the beginning (1.5 +/- 1.05 damage index - DI) or 10 days after the placement of the orthodontic appliance (2.5 +/- 3.08 DI) and did not change significantly between these time points (p= 0.0913). Conversely, there was a significant increase in MN frequency 30 days after the beginning of the treatment (p= 0.0236). In this study, the MN assay was shown to be more sensitive than the CA. Other investigations are necessary in order to assess the genotoxic potential of orthodontic fixed appliances associated with long-term studies concerning these effects in orthodontic patients.
DOI：
10.1016/j.mrrev.2008.03.007
发表时间：
2008-07-01
影响因子：
5.3
作者：
Holland, Nina;Bolognesi, Claudia;Fenech, Michael
通讯作者：
Fenech, Michael
DOI：
10.1016/0165-1161(94)90007-8
发表时间：
1994-02-01
期刊：
MUTATION RESEARCH
影响因子：
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作者：
TITENKOHOLLAND, N;MOORE, LE;SMITH, MT
通讯作者：
SMITH, MT