Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.
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DOI：
10.1371/journal.pone.0064822
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Pleshko N
中科院分区：
文献类型：
--
作者：
Hanifi A;McCarthy H;Roberts S;Pleshko N
在修复关节软骨时，经常发现由混合胶原类型组成的透明软骨和机械性较差的纤维软骨。本研究旨在开发方法，以确定胶原蛋白类型和其他组织成分，使用傅立叶变换红外（FTIR）光谱评价基质成分结合多变量分析。修复软骨，I型和II型胶原蛋白，和聚集蛋白聚糖的主要分子组分的FTIR光谱，被用来开发多元光谱模型的歧视感兴趣的组织的基质成分。从牛骨、肌腱、正常软骨、半月板和人修复软骨组织中收集红外成像数据，并使用偏最小二乘分析预测组合物。组织学和免疫组织化学结果用作验证标准。还从半月板（具有混合胶原类型的组织）获得红外光纤探针光谱数据，以评估该方法在微创临床应用中用于识别胶原类型的潜力。从多变量分析中获得的组织成分的浓度分布与组织学和免疫组织化学结果非常一致。骨和肌腱显示出均匀分布的主要是I型胶原通过组织。正常软骨显示II型胶原蛋白和蛋白多糖的分布类似于已知的组合物，而在修复软骨，I型和II型胶原蛋白的光谱分布类似于通过免疫组织化学观察到的。使用探针，半月板的外部和内部区域被证明是主要由I型和II型胶原蛋白，分别根据免疫组织化学数据。总之，红外光谱的多变量分析确实可以用于区分胶原蛋白I型和II型，即使在蛋白聚糖的存在下，在结缔组织中，使用成像和光纤方法。这对于监测组织修复的临床原位应用具有巨大潜力。
Hyaline cartilage and mechanically inferior fibrocartilage consisting of mixed collagen types are frequently found together in repairing articular cartilage. The present study seeks to develop methodology to identify collagen type and other tissue components using Fourier transform infrared (FTIR) spectral evaluation of matrix composition in combination with multivariate analyses. FTIR spectra of the primary molecular components of repair cartilage, types I and II collagen, and aggrecan, were used to develop multivariate spectral models for discrimination of the matrix components of the tissues of interest. Infrared imaging data were collected from bovine bone, tendon, normal cartilage, meniscus and human repair cartilage tissues, and composition predicted using partial least squares analyses. Histology and immunohistochemistry results were used as standards for validation. Infrared fiber optic probe spectral data were also obtained from meniscus (a tissue with mixed collagen types) to evaluate the potential of this method for identification of collagen type in a minimally-invasive clinical application. Concentration profiles of the tissue components obtained from multivariate analysis were in excellent agreement with histology and immunohistochemistry results. Bone and tendon showed a uniform distribution of predominantly type I collagen through the tissue. Normal cartilage showed a distribution of type II collagen and proteoglycan similar to the known composition, while in repair cartilage, the spectral distribution of both types I and II collagen were similar to that observed via immunohistochemistry. Using the probe, the outer and inner regions of the meniscus were shown to be primarily composed of type I and II collagen, respectively, in accordance with immunohistochemistry data. In summary, multivariate analysis of infrared spectra can indeed be used to differentiate collagen type I and type II, even in the presence of proteoglycan, in connective tissues, using both imaging and fiber optic methodology. This has great potential for clinical in situ applications for monitoring tissue repair.
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影响因子：
3.8
作者：
Gao, JZ
通讯作者：
Gao, JZ
DOI：
10.1186/ar380
发表时间：
2002
期刊：
Arthritis research
影响因子：
--
作者：
Eyre D
通讯作者：
Eyre D
影响因子：
--
作者：
Hollander, Anthony P.;Dickinson, Sally C.;Abatangelo, Giovanni
通讯作者：
Abatangelo, Giovanni
影响因子：
15.9
作者：
DODGE, GR;POOLE, AR
通讯作者：
POOLE, AR
用于软骨组织工程的定向软骨细胞外基质衍生支架
影响因子：
2.8
作者：
Jia, Shuaijun;Liu, Lie;Liu, Jian
通讯作者：
Liu, Jian