Vesicular ATPase inserted into the plasma membrane of motor terminals by exocytosis alkalinizes cytosolic pH and facilitates endocytosis.
Vesicular ATPase inserted into the plasma membrane of motor terminals by exocytosis alkalinizes cytosolic pH and facilitates endocytosis.
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DOI：
10.1016/j.neuron.2010.11.035
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发表时间：
2010-12-22
期刊：
影响因子：
16.2
通讯作者：
David, Gavriel
中科院分区：
文献类型：
--
作者：
Zhang, Zhongsheng;Nguyen, Khanh T.;Barrett, Ellen F.;David, Gavriel
胞浆pH值对囊泡神经递质释放的关键成分如Ca ~（2+）内流和膜再循环有影响。我们测量了转基因表达的黄色荧光蛋白在小鼠运动神经末梢中的pH敏感性荧光，并报告了动作电位序列引起的Ca ~（2+）内流（12.5-100 Hz）引起双相pH变化：短暂酸化（[H+]平均峰值增加约13 nM），随后是长时间碱化（[H+]峰值降低约30 nM），其持续时间超过刺激序列。通过用肉毒杆菌神经毒素阻断囊泡胞吐作用选择性地消除碱化，并通过胞吞抑制剂dynasore延长碱化。通过囊泡H+-ATP酶（用福利霉素或巴弗洛霉素）阻断H+泵送抑制刺激诱导的碱化并减少FM 1 -43的内吞摄取。这些结果表明，H+-ATP酶，已知转移到预融合囊泡胞质H+，继续挤出胞质H+后被exocytotically纳入质膜。由此产生的细胞质碱化可能有利于囊泡内吞作用。
Key components of vesicular neurotransmitter release, such as Ca2+ influx and membrane recycling, are affected by cytosolic pH. We measured the pH-sensitive fluorescence of Yellow Fluorescent Protein transgenically expressed in mouse motor nerve terminals, and report that Ca2+ influx elicited by action potential trains (12.5-100 Hz) evokes a biphasic pH change: a brief acidification (~13 nM average peak increase in [H+]), followed by a prolonged alkalinization (~30 nM peak decrease in [H+]) which outlasts the stimulation train. The alkalinization is selectively eliminated by blocking vesicular exocytosis with botulinum neurotoxins, and is prolonged by the endocytosis-inhibitor dynasore. Blocking H+ pumping by vesicular H+-ATPase (with folimycin or bafilomycin) suppresses stimulation-induced alkalinization and reduces endocytotic uptake of FM1-43. These results suggest that H+-ATPase, known to transfer cytosolic H+ into pre-fused vesicles, continues to extrude cytosolic H+ after being exocytotically incorporated into the plasma membrane. The resulting cytosolic alkalinization may facilitate vesicular endocytosis.
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影响因子：
4
作者：
Bolshakov, Alexey P.;Mikhailova, Maria M.;Khodorov, Boris I.
通讯作者：
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影响因子：
25
作者：
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DOI：
10.1098/rspb.1979.0095
发表时间：
1979-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
ATTWELL, D;ILES, JF
通讯作者：
ILES, JF
影响因子：
5.3
作者：
Gaffield, Michael A.;Tabares, Lucia;Betz, William J.
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影响因子：
7.3
作者：
Katz, E;Protti, DA;Uchitel, OD
通讯作者：
Uchitel, OD