A Novel Human Bone Marrow Stroma-Derived Cell Line TF274 Is Highly Osteogenic In Vitro and In Vivo
A Novel Human Bone Marrow Stroma-Derived Cell Line TF274 Is Highly Osteogenic In Vitro and In Vivo
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新型人骨髓基质衍生细胞系 TF274 在体外和体内均具有高度成骨作用
DOI：
10.1007/s002239900517
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发表时间：
1998
影响因子：
4.2
通讯作者：
M. Gowen
中科院分区：
文献类型：
--
作者：
U. Prabhakar;I. James;R. Dodds;E. Lee;D. Rieman;D. Lipshutz;S. Trulli;Z. Jonak;K. Tan;F. Drake;M. Gowen
描述了一种新的永生化的人骨髓基质来源的细胞系TF274，它在体外和体内都具有成骨的能力。在基础条件下，这些细胞表达碱性磷酸酶(ALP)和I型胶原基因，这是成骨细胞表型的特征。在促骨药如甲状旁腺激素、转化生长因子β-β和骨形态发生蛋白-2的存在下，碱性磷酸酶水平升高。此外，甲状旁腺素还增加了这些细胞内cAMP的水平。在L-抗坏血酸和β-甘油磷酸钠存在下培养这些细胞，以评估它们在体外成骨的能力。茜素红染色检测细胞基质矿化，45Ca摄取增加。在此条件下，在不到2周的时间内观察到矿化结核的形成。对TF274细胞在矿化过程中不同时间的Northern分析表明，骨钙素基因的瞬时表达通常与成骨细胞的分化有关。使用严重联合免疫缺陷(SCID)小鼠模型在体内证实了TF274细胞的成骨特性。抗人类白细胞抗原、I类抗原和人Oka血型抗原的抗体被用来证明形成的病变是人类起源的。到第21天，损伤包括一个均匀的ALP阳性细胞灶，包含矿化骨区域，衬里有酒石酸抗性酸性磷酸酶(TRAP)阳性破骨细胞。因此，TF274细胞在体外和体内都显示出成骨潜力。这一永生化细胞系代表了一致的细胞来源，可用于研究人成骨细胞在体外和体内的分化。
A novel, immortalized, human bone marrow stroma-derived cell line TF274 is described which has the ability to form bone both in vitro and in vivo. Under basal conditions these cells expressed alkaline phosphatase (ALP) and type I collagen genes which are characteristic of the osteoblast phenotype. ALP levels were upregulated in the presence of osteotropic agents such as parathyroid hormone (PTH), transforming growth factor beta (TGF-β), and BMP-2. In addition, PTH also increased cAMP levels in these cells. The capacity of these cells to form bone in vitro was evaluated by culturing them in the presence of L-ascorbic acid and β-glycerophosphate. Matrix mineralization in these cultures was assessed by Alizarin Red staining and increased 45Ca uptake. Under these conditions mineralized nodule formation was observed in less than 2 weeks. Northern analysis of TF274 cells at various times during the mineralization process indicated a temporal expression of the osteocalcin gene that is typically associated with differentiating osteoblasts. The osteogenic nature of TF274 cells was confirmed in vivo using the severe combined immunodeficient (SCID) mouse model. Antibodies to human leukocyte antigens (HLA), class I antigens, and human OKa blood group antigen were used to demonstrate that the lesions formed were of human origin. By 21 days, the lesion consisted of a homogeneous focus of ALP-positive cells containing areas of mineralized bone lined with tartarate-resistant acid phosphatase (TRAP) positive osteoclasts. Thus, the TF274 cells exhibit osteogenic potential both in vitro and in vivo. This immortalized cell line represents a consistent source of cells that can be used to study human osteoblast differentiation both in vitro and in vivo.
从人胎儿颅骨中分离和培养成骨细胞祖细胞。
影响因子：
0.9
作者：
Eastlund,T;Low,WC;Mooradian,DL
通讯作者：
Mooradian,DL
DOI：
10.1016/s0021-9258(18)34781-1
发表时间：
1982-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Majeska;G. Rodan
通讯作者：
R. Majeska;G. Rodan