Toxoplasma GRA Peptide-Specific Serologic Fingerprints Discriminate Among Major Strains Causing Toxoplasmosis.
Toxoplasma GRA Peptide-Specific Serologic Fingerprints Discriminate Among Major Strains Causing Toxoplasmosis.
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弓形虫 GRA 肽特异性血清学指纹可区分引起弓形虫病的主要菌株。
DOI：
10.3389/fcimb.2021.621738
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发表时间：
2021
影响因子：
5.7
通讯作者：
Saeij JPJ
中科院分区：
文献类型：
--
作者：
Arranz-Solís D;Carvalheiro CG;Zhang ER;Grigg ME;Saeij JPJ
弓形虫病的严重程度取决于宿主和寄生虫因素的组合。其中，引起感染的弓形虫菌株是疾病结局的重要决定因素。2型菌株在欧洲占主导地位，而在北美2型，其次是3型和12型菌株通常从野生动物和患者中分离出来。为了确定一个人感染的菌株类型，血清学分型提供了一个有前途的替代方案，往往是危险的，并不总是可能的活检为基础的DNA基因分型方法。然而，尽管最近在血清分型方面取得了进展，但仍然需要提高灵敏度和特异性，并且它还不能区分感染人群的主要弓形虫谱系。此外，由于非1/2/3菌株引起的感染与更严重的疾病有关，因此识别这些菌株的能力至关重要。在本研究中，我们研究了基于ELISA的检测方法的诊断潜力，该方法使用来自最近大规模肽阵列筛选的28种免疫原性弓形虫肽。我们的研究结果表明，一个离散的肽，来自弓形虫致密颗粒蛋白（GRA 3，GRA 5，GRA 6和GRA 7）是足以区分感染小鼠和人类的原型菌株。该测定特别依赖于比较个体血清反应性与GRA特异性多态性肽变体的比率，以确定每种主要菌株的“反应性指纹”。重要的是，具有不同于1/2/3型的独特等位基因组合的非原型菌株显示出非反应性或不同组合的混合血清反应性特征，其本身具有诊断性，并且可用于鉴定这些菌株。值得注意的是，我们使用GRA 6肽鉴定了独特的“HG 11/12”反应性模式，其能够将HG 11/12与原型北美/欧洲菌株感染区分开。
The severity of toxoplasmosis depends on a combination of host and parasite factors. Among them, the Toxoplasma strain causing the infection is an important determinant of the disease outcome. Type 2 strains dominate in Europe, whereas in North America type 2, followed by type 3 and 12 strains are commonly isolated from wildlife and patients. To identify the strain type a person is infected with, serological typing provides a promising alternative to the often risky and not always possible biopsy-based DNA methods of genotyping. However, despite recent advances in serotyping, improvements in the sensitivity and specificity are still needed, and it does not yet discriminate among the major Toxoplasma lineages infecting people. Moreover, since infections caused by non-1/2/3 strains have been associated with more severe disease, the ability to identify these is critical. In the present study we investigated the diagnostic potential of an ELISA-based assay using 28 immunogenic Toxoplasma peptides derived from a recent large-scale peptide array screen. Our results show that a discrete number of peptides, derived from Toxoplasma dense granule proteins (GRA3, GRA5, GRA6, and GRA7) was sufficient to discriminate among archetypal strains that infect mice and humans. The assay specifically relies on ratios that compare individual serum reactivities against GRA-specific polymorphic peptide variants in order to determine a “reactivity fingerprint” for each of the major strains. Importantly, nonarchetypal strains that possess a unique combination of alleles, different from types 1/2/3, showed either a non-reactive, or different combinatorial, mixed serum reactivity signature that was diagnostic in its own right, and that can be used to identify these strains. Of note, we identified a distinct “HG11/12” reactivity pattern using the GRA6 peptides that is able to distinguish HG11/12 from archetypal North American/European strain infections.
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影响因子：
5.7
作者：
Arranz-Solis, David;Cordeiro, Cynthia;Saeij, Jeroen P. J.
通讯作者：
Saeij, Jeroen P. J.
影响因子：
4
作者：
Dubey, J. P.;Velmurugan, G. V.;Su, C.
通讯作者：
Su, C.
影响因子：
4.4
作者：
Herrmann DC;Bärwald A;Maksimov A;Pantchev N;Vrhovec MG;Conraths FJ;Schares G
通讯作者：
Schares G
影响因子：
3.7
作者：
Maksimov P;Zerweck J;Maksimov A;Hotop A;Gross U;Spekker K;Däubener W;Werdermann S;Niederstrasser O;Petri E;Mertens M;Ulrich RG;Conraths FJ;Schares G
通讯作者：
Schares G
影响因子：
9.4
作者：
Howe, DK;Honore, S;Sibley, LD
通讯作者：
Sibley, LD