Toxoplasma gondii sexual cross in a single naturally infected feline host: generation of highly mouse-virulent and avirulent clones, genotypically different from clonal types I, II and III.
Toxoplasma gondii sexual cross in a single naturally infected feline host: generation of highly mouse-virulent and avirulent clones, genotypically different from clonal types I, II and III.
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DOI：
10.1186/1297-9716-43-39
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发表时间：
2012-04-30
影响因子：
4.4
通讯作者：
Schares G
中科院分区：
文献类型：
--
作者：
Herrmann DC;Bärwald A;Maksimov A;Pantchev N;Vrhovec MG;Conraths FJ;Schares G
从一个单一的弓形虫卵囊现场样品获得的速殖子克隆基因型和小鼠毒力的特点。用9个独立的、不连锁的遗传标记nSAG 2、SAG 3、BTUB、GRA 6、c22-8、c29-2、L358、PK 1和Apico对从干扰素-γ-敲除（GKO）小鼠、BALB/c小鼠和VERO细胞培养物中分离的速殖子进行PCR-RFLP基因分型，结果显示混合T.在不同基因座显示II型和III型等位基因组合的弓形虫感染。从所有的混合T.有限稀释法培养弓形虫速殖子。十六T。弓形虫克隆在所有位点均显示III型等位基因，29个克隆在不同位点显示II型和III型等位基因的组合。共鉴定出5个克隆群，其中4个包括T。显示非典型等位基因模式的弓形虫克隆，以前从未在自然感染中描述过。除两个外，所有测试的克隆在BALB/c小鼠中均具有高毒性。分离不同的非典型T。从一只自然感染的猫的卵囊样品中获得的弓形虫克隆表明，通过T.弓形虫在自然条件下发生，并导致小鼠中出现毒力增加的克隆。
Tachyzoite clones obtained from a single Toxoplasma gondii oocyst field sample were genotyped and characterized regarding mouse virulence. PCR-RFLP genotyping of tachyzoites initially isolated from interferon-γ-knockout (GKO) mice, BALB/c mice and VERO cell culture using the nine independent, unlinked genetic markers nSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico revealed mixed T. gondii infections showing combinations of type II and type III alleles at different loci. Forty-five individual clones were obtained from all mixed T. gondii tachyzoite cell cultures by limiting dilution. Sixteen T. gondii clones showed type III alleles at all loci and 29 clones displayed a combination of type II and type III alleles at different loci. Five clone groups were identified in total, four of which include T. gondii clones that showed a non-canonical allele pattern and have never been described in natural infections before. All tested clones, except two, were highly virulent in BALB/c mice. The isolation of different non-canonical T. gondii clones originating from an oocyst sample of a single naturally infected cat demonstrate that sexual recombination as well as re-assortment of chromosomes via a sexual cross of T. gondii occur under natural conditions and result in the emergence of clones with increased virulence in mice.
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影响因子：
11.8
作者：
de Moura L;Bahia-Oliveira LM;Wada MY;Jones JL;Tuboi SH;Carmo EH;Ramalho WM;Camargo NJ;Trevisan R;Graça RM;da Silva AJ;Moura I;Dubey JP;Garrett DO
通讯作者：
Garrett DO
影响因子：
2.6
作者：
Dubey, J. P.;Patitucci, A. N.;Shen, S. K.
通讯作者：
Shen, S. K.
影响因子：
4
作者：
Herrmann, D. C.;Pantchev, N.;Schares, G.
通讯作者：
Schares, G.
影响因子：
2.6
作者：
de Sousa, S;Ajzenberg, D;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
2.6
作者：
Dubey, J. P.;Sundar, N.;Su, C.
通讯作者：
Su, C.