Transformation and morphological changes of murine L cells by transfection with a mutated form of beta-catenin.
Transformation and morphological changes of murine L cells by transfection with a mutated form of beta-catenin.
复制标题
通过用突变形式的 β-连环蛋白转染小鼠 L 细胞的转化和形态学变化。
作者：
Y. Nagasawa;Y. Miyoshi;K. Iwao;Y. Shinomura;Y. Matsuzawa;Y. Nakamura
为了阐明突变β -连环蛋白的致癌性质，我们将一种缺乏完整外显子3的cDNA形式引入来自小鼠s.c.组织的L细胞中。异常β -连环蛋白在这些细胞的细胞质和细胞核中积累（称为L- mt），而在转染野生型β -连环蛋白（称为L- n）的L细胞中，正常β -连环蛋白的表达水平与亲本细胞相似。L-MT细胞在形态上也从成纤维细胞样的外观转变为更立方体的形状。它们的增殖速度与L细胞和L- n细胞相同，但L- mt细胞的饱和密度似乎随着多层生长模式的增加而增加。此外，与亲代细胞相比，L-MT细胞在生长培养基中需要较低浓度的血清。这些细胞生长和形态的改变表明突变的β -连环蛋白在转染细胞中稳定并诱导了致癌表型。
To shed light on the oncogenic nature of mutant beta-catenin, we introduced a form of the cDNA that lacked an entire exon 3 into L cells derived from murine s.c. tissue. Aberrant beta-catenin protein accumulated in the cytoplasm and nuclei of these cells (designated L-MT), whereas in L cells transfected with wild-type beta-catenin (designated L-N), normal beta-catenin protein was expressed at a level similar to that of parental cells. L-MT cells also changed morphologically from a fibroblast-like appearance to a more cuboidal shape. Their rate of proliferation was the same as that of L cells and L-N cells, but the saturation density of L-MT cells appeared to increase in association with a multilayer growth pattern. Furthermore, L-MT cells required a lower concentration of serum in the growth medium than did parental cells. These alterations in cell growth and morphology suggested that mutated beta-catenin was stabilized in the transfected cells and induced the oncogenic phenotype.
影响因子：
10.5
作者：
Yost, C;Torres, M;Moon, RT
通讯作者：
Moon, RT
影响因子：
3.3
作者：
Fischer, T.;Guimera, J.;Prakash, N.
通讯作者：
Prakash, N.