High-Throughput Assessment of Cellular Mechanical Properties.
High-Throughput Assessment of Cellular Mechanical Properties.
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DOI：
10.1146/annurev-bioeng-071114-040545
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发表时间：
2015
影响因子：
9.7
通讯作者：
Di Carlo D
中科院分区：
文献类型：
--
作者：
Darling EM;Di Carlo D
传统上，细胞分析的重点是使用分子生物标志物进行基础研究，细胞制备和临床诊断;然而，新的微技术正在使细胞的机械性能评估的吞吐量，使他们适合广泛使用。我们回顾了目前对细胞的机械特性如何与潜在的分子和结构变化相关的理解，描述了这些变化如何随着细胞状态和疾病过程而演变，并提出了有前途的生物医学应用，这些应用将通过增加机械测试的吞吐量来促进：从诊断癌症和监测免疫状态到为再生医学准备细胞。我们提供的背景技术奠定了定量理解细胞力学的基础，并讨论目前的努力，以开发强大的技术，旨在实现机械表型作为一种常规工具，在生物医学的快速分析。展望未来，我们描述了将促进广泛采用的其他里程碑，以及不仅在机械评估细胞方面而且在扰动它们以被动地设计细胞状态方面的新方向。
Traditionally, cell analysis has focused on using molecular biomarkers for basic research, cell preparation, and clinical diagnostics; however, new microtechnologies are enabling evaluation of the mechanical properties of cells at throughputs that make them amenable to widespread use. We review the current understanding of how the mechanical characteristics of cells relate to underlying molecular and architectural changes, describe how these changes evolve with cell-state and disease processes, and propose promising biomedical applications that will be facilitated by the increased throughput of mechanical testing: from diagnosing cancer and monitoring immune states to preparing cells for regenerative medicine. We provide background about techniques that laid the groundwork for the quantitative understanding of cell mechanics and discuss current efforts to develop robust techniques for rapid analysis that aim to implement mechanophenotyping as a routine tool in biomedicine. Looking forward, we describe additional milestones that will facilitate broad adoption, as well as new directions not only in mechanically assessing cells but also in perturbing them to passively engineer cell state.
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影响因子：
8.6
作者：
ASHKIN, A
通讯作者：
ASHKIN, A
影响因子：
4.4
作者：
Chan CJ;Whyte G;Boyde L;Salbreux G;Guck J
通讯作者：
Guck J
影响因子：
7.4
作者：
Adamo, Andrea;Arione, Alessandro;Sharei, Armon;Jensen, Klavs F.
通讯作者：
Jensen, Klavs F.
影响因子：
3.7
作者：
Auburn S;Marfurt J;Maslen G;Campino S;Ruano Rubio V;Manske M;Machunter B;Kenangalem E;Noviyanti R;Trianty L;Sebayang B;Wirjanata G;Sriprawat K;Alcock D;Macinnis B;Miotto O;Clark TG;Russell B;Anstey NM;Nosten F;Kwiatkowski DP;Price RN
通讯作者：
Price RN
影响因子：
3.8
作者：
Applegate, RW;Squier, J;Marr, DWM
通讯作者：
Marr, DWM