New live screening of plant-nematode interactions in the rhizosphere.
New live screening of plant-nematode interactions in the rhizosphere.
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在根际中植物 - 纽面相互作用的新实时筛选。
DOI：
10.1038/s41598-017-18797-7
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发表时间：
2018-01-23
影响因子：
4.6
通讯作者：
Dupuy LX
中科院分区：
文献类型：
--
作者：
O'Callaghan FE;Braga RA;Neilson R;MacFarlane SA;Dupuy LX
自由生活线虫（FLN）是在所有土壤中发现的微小蠕虫。虽然许多FLN物种对作物有益，但有些物种通过取食根部和携带病毒造成重大损害。随着计划立法取消传统使用的化学处理，确定管理FLN种群的新方法已成为高度优先事项。为此，需要更强大的筛选系统来快速评估对作物的威胁并有效地确定治疗方法。在这里，我们已经开发了新的活的检测方法，用于测试线虫的治疗反应相结合的透明土壤微宇宙，一种新的光片成像技术，称为生物斑点选择性平面照明显微镜（BSPIM）的快速线虫检测，和共聚焦激光扫描显微镜的高分辨率成像。我们表明，BSPIM增加信噪比高达60倍，并允许自动检测FLN在1.5毫升的透明土壤样品。生长植物根系迅速扫描线虫的丰度和活动，FLN喂养行为和化学化合物在土壤样条件下观察到的反应。这种方法可用于直接监测FLN活性，以开发针对经济上具有破坏性的食草线虫的新化合物，或确保有益物种不会受到负面影响。
Free living nematodes (FLN) are microscopic worms found in all soils. While many FLN species are beneficial to crops, some species cause significant damage by feeding on roots and vectoring viruses. With the planned legislative removal of traditionally used chemical treatments, identification of new ways to manage FLN populations has become a high priority. For this, more powerful screening systems are required to rapidly assess threats to crops and identify treatments efficiently. Here, we have developed new live assays for testing nematode responses to treatment by combining transparent soil microcosms, a new light sheet imaging technique termed Biospeckle Selective Plane Illumination Microscopy (BSPIM) for fast nematode detection, and Confocal Laser Scanning Microscopy for high resolution imaging. We show that BSPIM increased signal to noise ratios by up to 60 fold and allowed the automatic detection of FLN in transparent soil samples of 1.5 mL. Growing plant root systems were rapidly scanned for nematode abundance and activity, and FLN feeding behaviour and responses to chemical compounds observed in soil-like conditions. This approach could be used for direct monitoring of FLN activity either to develop new compounds that target economically damaging herbivorous nematodes or ensuring that beneficial species are not negatively impacted.
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影响因子：
3.9
作者：
Adamiak, Anna;Zdunek, Artur;Rutkowski, Krzysztof
通讯作者：
Rutkowski, Krzysztof
影响因子：
1.3
作者：
Arizaga, R;Cap, NL;Trivi, M
通讯作者：
Trivi, M
DOI：
10.1073/pnas.0804612105
发表时间：
2008-08-05
影响因子：
11.1
作者：
Cui, Xiquan;Lee, Lap Man;Yang, Changhuei
通讯作者：
Yang, Changhuei
影响因子：
2.4
作者：
BRIERS, JD
通讯作者：
BRIERS, JD
影响因子：
9.2
作者：
Choe, Andrea;von Reuss, Stephan H.;Kogan, Dima;Gasser, Robin B.;Platzer, Edward G.;Schroeder, Frank C.;Sternberg, Paul W.
通讯作者：
Sternberg, Paul W.