Ascaroside signaling is widely conserved among nematodes.
Ascaroside signaling is widely conserved among nematodes.
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DOI：
10.1016/j.cub.2012.03.024
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发表时间：
2012-05-08
期刊：
影响因子：
9.2
通讯作者：
Sternberg, Paul W.
中科院分区：
文献类型：
--
作者：
Choe, Andrea;von Reuss, Stephan H.;Kogan, Dima;Gasser, Robin B.;Platzer, Edward G.;Schroeder, Frank C.;Sternberg, Paul W.
线虫是地球上最成功的动物之一，包括重要的人类病原体，但对线虫信息素系统知之甚少。已发现一组称为ascarosides的小分子介导秀丽隐杆线虫中的交配发现、聚集和发育滞育，但不清楚ascarosides信号传导是否存在于隐杆线虫属之外。为了确定是否被其他线虫物种用作信号分子，我们进行了一个基于质谱的屏幕从各种自由生活和寄生（植物，昆虫和动物）线虫分泌物中的蛔虫。我们发现，大多数分析的物种，包括线虫从几个不同的分支，产生物种特异性的蛔虫苷混合物。在某些情况下，Ascaroside的生物合成模式似乎与生殖相关，而在其他情况下，生物合成似乎与生活方式和生态位相关。我们进一步表明，Ascarosides介导不同的线虫行为，例如保留、回避和远程吸引，并且不同的线虫物种对不同但重叠的Ascarosides组有反应。我们的研究结果表明，线虫利用一个保守的家庭的信号分子，尽管已经进化到占据不同的生态。它们的结构特征和保守性水平令人想起细菌群体感应，其中酰基高丝氨酸内酯（AHLs）由许多种革兰氏阴性细菌产生和感应。物种特异性蛔虫苷谱的鉴定可能使基于信息素的方法能够干扰寄生线虫的繁殖和存活，寄生线虫是造成全球重大农业损失和许多人类疾病的原因。
Nematodes are among the most successful animals on earth and include important human pathogens, yet little is known about nematode pheromone systems. A group of small molecules called ascarosides has been found to mediate mate finding, aggregation, and developmental diapause in Caenorhabditis elegans, but it is unknown whether ascaroside signaling exists outside of the genus Caenorhabditis. To determine whether ascarosides are used as signaling molecules by other nematode species, we performed a mass spectrometry-based screen for ascarosides in secretions from a variety of both free-living and parasitic (plant, insect, and animal) nematodes. We found that most of the species analyzed, including nematodes from several different clades, produce species-specific ascaroside mixtures. In some cases, ascaroside biosynthesis patterns appear to correlate with phylogeny, whereas in other cases, biosynthesis seems to correlate with lifestyle and ecological niche. We further show that ascarosides mediate distinct nematode behaviors, such as retention, avoidance, and long-range attraction, and that different nematode species respond to distinct, but overlapping, sets of ascarosides. Our findings indicate that nematodes utilize a conserved family of signaling molecules despite having evolved to occupy diverse ecologies. Their structural features and level of conservation are evocative of bacterial quorum sensing, where acyl homoserine lactones (AHLs) are both produced and sensed by many species of Gram-negative bacteria. The identification of species-specific ascaroside profiles may enable pheromone-based approaches to interfere with reproduction and survival of parasitic nematodes, which are responsible for significant agricultural losses and many human diseases worldwide.
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影响因子：
30.8
作者：
Andersen, Erik C.;Gerke, Justin P.;Shapiro, Joshua A.;Crissman, Jonathan R.;Ghosh, Rajarshi;Bloom, Joshua S.;Felix, Marie-Anne;Kruglyak, Leonid
通讯作者：
Kruglyak, Leonid
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作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
Paik, YK