Identification and gene expression analysis of serine proteases and their homologs in the Asian corn borer Ostrinia furnacalis.
Identification and gene expression analysis of serine proteases and their homologs in the Asian corn borer Ostrinia furnacalis.
复制标题
丝氨酸蛋白酶及其同源物中的鉴定和基因表达分析。
DOI：
10.1038/s41598-023-31830-2
复制
发表时间：
2023-03-23
影响因子：
4.6
通讯作者：
Xu, Junfeng
中科院分区：
文献类型：
--
作者：
Yang, Lei;Xu, Xiaoli;Wei, Wei;Chen, Xiaoyun;Peng, Cheng;Wang, Xiaofu;Xu, Junfeng
丝氨酸蛋白酶（SP）及其同源物（SPH）是最具特征的基因家族之一。它们参与几个生理过程，包括消化、胚胎发育和免疫。在本研究中，共有177个SPs相关基因的基因组中的奥斯特里尼亚mesacalis。对SPs/SPHs的活化位点和SPs的酶特异性进行了鉴定，结果表明，所分析的SPs大多具有胰蛋白酶底物特异性。几个具有相似简单基因结构的SP/SPH在支架上呈串联重复样分布，表明该大家族中发生了基因扩增。此外，我们构建了30个RNA测序文库，包括4个发育期和4个中期幼虫组织，以研究这些基因的转录水平。通过数据分析获得差异上调和下调的基因。超过四分之一的基因被特别鉴定为在中肠中高度表达，与其他三个组织相比。本研究对O.探索了棘皮动物。模式昆虫与O.指出了它们可能的功能。这些信息为理解这个大家庭的功能角色提供了基础。
Serine proteases (SPs) and their homologs (SPHs) are among the best-characterized gene families. They are involved in several physiological processes, including digestion, embryonic development and immunity. In the current study, a total of 177 SPs-related genes were characterized in the genome of Ostrinia furnacalis. The activation site of SPs/SPHs and enzyme specificity of SPs were identified, and the findings showed that most of the SPs analyzed possessed trypsin substrate specificity. Several SPs/SPHs with similar simple gene structures had tandem repeat-like distributions on the scaffold, indicated that gene expansion has occurred in this large family. Furthermore, we constructed 30 RNA sequencing libraries including four with developmental stage and four middle larval stage tissues to study the transcript levels of these genes. Differentially upregulated and downregulated genes were obtained via data analysis. More than one-quarter of the genes were specifically identified as highly expressed in the midgut in compared to the other three tissues evaluated. In the current study, the domain structure, gene location and phylogenetic relationship of genes in O. furnacalis were explored. Orthologous comparisons of SPs/SPHs between model insects and O. furnacalis indicated their possible functions. This information provides a basis for understanding the functional roles of this large family.
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DOI：
10.1046/j.1432-1033.2002.03155.x
发表时间：
2002-09-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Lee, KY;Zhang, R;Lee, BL
通讯作者：
Lee, BL
影响因子：
4.1
作者：
Afidchao, Miladis M.;Musters, C. J. M.;de Snoo, Geert R.
通讯作者：
de Snoo, Geert R.
影响因子：
3.8
作者：
Cao X;He Y;Hu Y;Zhang X;Wang Y;Zou Z;Chen Y;Blissard GW;Kanost MR;Jiang H
通讯作者：
Jiang H
DOI：
10.1073/pnas.90.11.4937
发表时间：
1993-06-01
影响因子：
11.1
作者：
APPEL, LF;PROUT, M;FRISTROM, J
通讯作者：
FRISTROM, J
影响因子：
4.8
作者：
Kim, MS;Baek, MJ;Lee, BL
通讯作者：
Lee, BL