Analysis of the heterogeneity of the BCR H-CDR3 repertoire in the bone marrow and spleen of 3-, 12-, and 20-month old mice.
Analysis of the heterogeneity of the BCR H-CDR3 repertoire in the bone marrow and spleen of 3-, 12-, and 20-month old mice.
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3、12、20月龄小鼠骨髓和脾脏中BCR H-CDR3库的异质性分析
DOI：
10.1186/s12979-021-00231-2
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发表时间：
2021-04-12
期刊：
影响因子：
--
通讯作者：
Yao X
中科院分区：
文献类型：
--
作者：
Ma L;Tao X;He X;Wang P;Ma L;Shi B;Yao X
老年小鼠中央和外周B细胞的数量及其反应性下降。随着年龄的增长，小鼠中央和外周B细胞库的多样性尚未得到阐明。在这项研究中，我们证明了在3、12和20月龄小鼠的骨髓B细胞、脾脏B细胞和脾脏记忆B细胞的BCR H-CDR3库中，某些v、D和jgenes的使用存在显著差异。在生产性序列、假基因序列和外框序列中，骨髓B细胞的5'J修剪随年龄的增长有显著差异；外周血脾B细胞和记忆B细胞在N1插入、N2插入、p5d插入和5d修剪方面随年龄的增长存在显著差异。小鼠骨髓B细胞、脾脏B细胞和脾脏记忆B细胞的BCR H-CDR3库多样性随着年龄的增长而降低。不同年龄小鼠骨髓和脾脏B细胞重叠比例在3月龄时低于12月龄和20月龄时，但脾脏记忆B细胞不重叠。本研究首次报道了随着小鼠年龄的增长，中央和外周B细胞CDR3库的同质性和异质性的变化，以进一步研究年轻/中年/老年小鼠B细胞免疫的下降和应答。
The number of central and peripheral B cells and their responsiveness are decreased in aged mice. The diversity of mice central and peripheral B cell repertoires with increasing age has not been elucidated. In this study, we demonstrated that there were significant differences in the usage of someV,D, andJgenes in the BCR H-CDR3 repertoire of bone marrow B cells, spleen B cells and spleen memory B cells in 3-, 12-, and 20-month-old mice. In the productive, pseudogene, and out-of-frame sequences, bone marrow B cells had significant differences in 5′J trimming with age; peripheral spleen B cells and memory B cells had significant differences in N1 insertion, N2 insertion, P5’D insertion, and 5’D trimming with age. The BCR H-CDR3 repertoire diversity of mice bone marrow B cells, spleen B cells and spleen memory B cells decreased with increasing age. The proportion of overlap in bone marrow and spleen B cells, but not spleen memory B cells, of mice at different ages was lower at 3 months than at 12 and 20 months. This study is the first to report the homogeneity and heterogeneity of the CDR3 repertoire of central and peripheral B cells change as mice age, to further investigation of the decline and response of B cell immunity in young/middle/old-aged mice.
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影响因子：
4.6
作者：
Shi B;Ma L;He X;Wu P;Wang P;Wang X;Ma R;Yao X
通讯作者：
Yao X
DOI：
10.1186/s12979-020-00210-z
发表时间：
2020-11-19
期刊：
Immunity & ageing : I & A
影响因子：
--
作者：
Frasca D;Blomberg BB
通讯作者：
Blomberg BB
影响因子：
32.4
作者：
Johnson, John L.;Rosenthal, Rebecca L.;Cancro, Michael P.
通讯作者：
Cancro, Michael P.
影响因子：
2
作者：
Pickman, Yishai;Dunn-Walters, Deborah;Mehr, Ramit
通讯作者：
Mehr, Ramit
影响因子：
7.3
作者：
Hong B;Wu Y;Li W;Wang X;Wen Y;Jiang S;Dimitrov DS;Ying T
通讯作者：
Ying T