Recovery from experimental rabies by adoptive transfer of immune cells.
Recovery from experimental rabies by adoptive transfer of immune cells.
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通过免疫细胞的过继转移从实验性狂犬病中恢复。
DOI：
10.1099/0022-1317-56-1-25
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Nathanson,N
中科院分区：
文献类型：
--
作者：
Prabhakar,BS;Fischman,HR;Nathanson,N
将狂犬病病毒Flury高卵传代（HEP）株脑内接种到成年小鼠中产生的短暂亚致死感染转化为致死感染（约100 mg/kg）。80 - 100%死亡率）。免疫抑制的感染动物对狂犬病没有免疫反应，并在感染后15至20天死亡。而在感染后第4天，用60 × 106个来自狂犬病免疫同基因供体的脾细胞过继免疫抑制小鼠，死亡率降低至12%。供体免疫后早期（4 ~ 11天）获得的淋巴细胞降低了死亡率，而晚期（免疫后16 ~ 32天）获得的淋巴细胞则无效。供体细胞保护动物的能力与供体细胞毒性T淋巴细胞（CTL）活性非常密切。免疫细胞转移后4天内，受体血清中和抗体和CTL水平与病毒感染的对照动物相当。免疫供体细胞分为胸腺来源的（富含T细胞）和骨髓来源的（富含B细胞）亚群。T和B亚群分别将死亡率降低至32%和34%。在这些动物中可以检测到CTL和血清中和抗体应答，尽管它们出现的时间晚于用未分级免疫脾细胞治疗的小鼠。目前的研究表明，B和T淋巴细胞需要狂犬病从中枢神经系统（CNS）的最佳清除，并建议狂犬病特异性CTLin体内的功能作用。
The transient, sublethal infection produced by intracerebral inoculation of the Flury high egg passage (HEP) strain of rabies virus into adult mice was converted into a lethal one (approx. 80 to 100% mortality) by administering 150 mg/kg cyclophosphamide (CY) 2 days after infection. Immunosuppressed, infected animals showed no immunological response to rabies and died 15 to 20 days after infection. However, mortality was reduced to 12% when suppressed mice were adoptively immunized, 4 days after infection, with an intravenous injection of 60 × 106spleen cells from rabies-immune syngeneic donors. The lymphocytes obtained early after donor immunization (4 to 11 days) reduced mortality, whereas those obtained late (16 to 32 days after immunization) were not effective. The ability of donor cells to protect animals corresponded very closely with donor cytotoxic T lymphocyte (CTL) activity. Within 4 days after immune cell transfer, serum neutralizing antibody and CTL levels in recipients were comparable to those found in virus-infected control animals.Immune donor cells were fractionated into thymus-derived (T-enriched) and bone marrow-derived (B-enriched) subsets. The T and B subsets reduced mortality to 32% and 34% respectively. CTL and serum neutralizing antibody responses could be detected in these animals, although they appeared later than in mice treated with unfractionated immune spleen cells. The present study demonstrates that both B and T lymphocytes are required for optimum clearance of rabies from the central nervous system (CNS) and suggests a functional role for rabies-specific CTLin vivo.
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组织培养中生长的狂犬病病毒的纯化
影响因子：
5.4
作者：
F. Sokol;E. Kuwert;T. Wiktor;K. Hummeler;H. Koprowski
通讯作者：
H. Koprowski
狂犬病疫苗的胸腺依赖性。
DOI：
10.1099/0022-1317-33-3-535
发表时间：
1976
期刊：
The Journal of general virology
影响因子：
--
作者：
G. Turner
通讯作者：
G. Turner
用于测定狂犬病中和抗体的快速可重复测试。
影响因子：
11.1
作者：
J. Smith;P. Yager;G. Baer
通讯作者：
G. Baer
抗体在实验性狂犬病恢复中的作用。
影响因子：
4.4
作者：
Aaron W. Miller;Herbert C. Morse;Jerry A. Winkelstein;Neal Nathanson
通讯作者：
Neal Nathanson
铝盐对培养的神经母细胞瘤细胞的影响
影响因子：
4.7
作者：
Carol A. Miller;Elliot M. Levine
通讯作者：
Elliot M. Levine