Growth characteristics of T-cell tropic HIV-1 vpu gene mutants in human peripheral blood mononuclear cells.
Growth characteristics of T-cell tropic HIV-1 vpu gene mutants in human peripheral blood mononuclear cells.
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T细胞嗜性HIV-1 vpu基因突变体在人外周血单核细胞中的生长特征。
DOI：
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发表时间：
1999
影响因子：
0.7
通讯作者：
A. Adachi
中科院分区：
文献类型：
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作者：
S. Iida;T. Fukumori;Y. Oshima;A. Koyama;A. Adachi
从人类免疫缺陷病毒1型(HIV-1)的嗜T细胞野生型(Wt)克隆中构建了一个缺乏完整VPU基因表达的突变体NL-E65，并对其在人类细胞中的复制特性进行了监测，同时还构建了一个表达C端截短VPU的突变体NL-SS。突变型NL-SS在两种细胞系和所有外周血单个核细胞(PBMC)中均有不同程度的生长，其生长动力学与野生型病毒相似。同样，突变体NL-E65在这两个细胞系和所有PBMC培养中都表现出VPU突变体的典型复制特性，在低水平生长。与先前报道的结果一起，这些数据表明，HIV-1 VPU对于病毒在迄今测试的任何类型的细胞中的复制都是必不可少的。
A mutant designated NL-E65, which lacks the expression of entire vpu gene, was constructed from T-cell tropic wild-type (wt) human immunodeficiency virus type 1 (HIV-1) clone and monitored for its replication property in human cells, along with a mutant NL-Ss which expresses a C-terminal truncated Vpu. The mutant NL-Ss could grow in two cell lines and in all peripheral blood mononuclear cell (PBMC) preparations to some extent, with kinetics similar to those of wt virus. Likewise, the mutant NL-E65 exhibited a replication property typical to the vpu mutant in the two cell lines and in all PBMC cultures, growing at a low level. Along with the results previously reported, these data indicate that HIV-1 Vpu is dispensable for virus replication in any of the types of cells so far tested.
DOI：
10.1073/pnas.86.13.5163
发表时间：
1989-07-01
影响因子：
11.1
作者：
TERWILLIGER, EF;COHEN, EA;HASELTINE, WA
通讯作者：
HASELTINE, WA