The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome.
The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome.
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DOI：
10.1186/gb-2012-13-3-r21
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发表时间：
2012
期刊：
影响因子：
12.3
通讯作者：
Cook KR
中科院分区：
文献类型：
--
作者：
Cook RK;Christensen SJ;Deal JA;Coburn RA;Deal ME;Gresens JM;Kaufman TC;Cook KR
染色体缺失广泛用于果蝇遗传学研究。删除图谱是用于精细基因定位的主要方法。有效且高效的缺失图谱需要广泛的基因组覆盖度和整个基因组中高密度的分子定义断点。布卢明顿果蝇库存中心的一个大型资源开发项目改进了删除的选择，超出了以前项目提供的范围。 FLP介导的带有FRT的转座子插入之间的重组被用来产生缺失，因为它是有效的并且在规划缺失筛选中提供单核苷酸分辨率。产生的 793 个缺失将常染色质基因组的覆盖率推至 98.4%。覆盖范围内的缺口包含单倍致死基因和单不育基因，但通过尽可能靠近这些基因的侧翼进行缺失，可以将这些缺口的大小最小化。为了提高覆盖率，生成了单倍致死和单倍不育基因的完整清单，并汇编了其他单倍不足基因的大量信息。为了帮助作图实验，将缺失的子集组织到缺陷试剂盒中，以有效地提供最大的覆盖范围。为了提高删除图谱的分辨率，计划进行筛选以在整个基因组中均匀分布删除断点。断点之间的中位染色体间隔现在仅包含 9 个基因，而 377 个间隔仅包含单个基因。果蝇现在拥有所有多细胞生物中最广泛的基因组缺失覆盖范围和断点细分，以及最全面的单倍体不足基因清单。染色体缺失菌株的改进选择将对几乎所有果蝇研究人员有用。
Chromosomal deletions are used extensively in Drosophila melanogaster genetics research. Deletion mapping is the primary method used for fine-scale gene localization. Effective and efficient deletion mapping requires both extensive genomic coverage and a high density of molecularly defined breakpoints across the genome. A large-scale resource development project at the Bloomington Drosophila Stock Center has improved the choice of deletions beyond that provided by previous projects. FLP-mediated recombination between FRT-bearing transposon insertions was used to generate deletions, because it is efficient and provides single-nucleotide resolution in planning deletion screens. The 793 deletions generated pushed coverage of the euchromatic genome to 98.4%. Gaps in coverage contain haplolethal and haplosterile genes, but the sizes of these gaps were minimized by flanking these genes as closely as possible with deletions. In improving coverage, a complete inventory of haplolethal and haplosterile genes was generated and extensive information on other haploinsufficient genes was compiled. To aid mapping experiments, a subset of deletions was organized into a Deficiency Kit to provide maximal coverage efficiently. To improve the resolution of deletion mapping, screens were planned to distribute deletion breakpoints evenly across the genome. The median chromosomal interval between breakpoints now contains only nine genes and 377 intervals contain only single genes. Drosophila melanogaster now has the most extensive genomic deletion coverage and breakpoint subdivision as well as the most comprehensive inventory of haploinsufficient genes of any multicellular organism. The improved selection of chromosomal deletion strains will be useful to nearly all Drosophila researchers.
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影响因子：
64.8
作者：
BERNSTEIN, SI;MOGAMI, K;EMERSON, CP
通讯作者：
EMERSON, CP
影响因子：
3.3
作者：
Cooper, Monica T.;Conant, Alexander W.;Kennison, James A.
通讯作者：
Kennison, James A.
影响因子：
3.3
作者：
Cook, R. Kimberley;Deal, Megan E.;Cook, Kevin R.
通讯作者：
Cook, Kevin R.
DOI：
10.1126/science.1179685
发表时间：
2009-11-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dickman DK;Davis GW
通讯作者：
Davis GW
DOI：
10.1083/jcb.200807052
发表时间：
2008-10-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Jang C;Lee G;Chung J
通讯作者：
Chung J