Carotenoid Biosynthetic Genes in Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.
Carotenoid Biosynthetic Genes in Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.
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作者：
Cao W;Wang P;Yang L;Fang Z;Zhang Y;Zhuang M;Lv H;Wang Y;Ji J
类胡萝卜素是由植物和微生物产生的天然功能色素，对人类健康起着至关重要的作用。甘蓝(Brassica Lenacea L.var.)Capitata L.)无论是生产还是消费，都是一种重要的经济作物。它营养丰富，含有β-胡萝卜素、叶黄素和其他抗氧化剂类胡萝卜素。在这里，我们系统地分析了全基因组上的类胡萝卜素生物合成基因，以了解甘蓝类胡萝卜素的生物合成途径。在甘蓝基因组中共鉴定出62个CBGs，它们是拟南芥47个CBGs的同源基因。在62个CBG中，46个甘蓝基因被定位到9条染色体上。对甘蓝、甜菜和甜菜类胡萝卜素生物合成同源基因对的进化分析表明，甘蓝的同源基因经历了与甘蓝相似的负选择。CBGs的表达分析表明，甘蓝和油菜的同源基因拷贝在功能上发生了分化。外源激素处理表明，ETH、ABA和MeJA能促进甘蓝中一些重要的CBGs的表达。系统发育分析表明，BoPSYs表现出高度的保守性。亚细胞定位分析表明，BoPSYs定位于叶绿体中。本研究首次对甘蓝类胡萝卜素生物合成基因进行了研究，为进一步研究甘蓝类胡萝卜素代谢机制奠定了基础。
Carotenoids are natural functional pigments produced by plants and microorganisms and play essential roles in human health. Cabbage (Brassica oleracea L. var. capitata L.) is an economically important vegetable in terms of production and consumption. It is highly nutritious and contains β-carotene, lutein, and other antioxidant carotenoids. Here, we systematically analyzed carotenoid biosynthetic genes (CBGs) on the whole genome to understand the carotenoid biosynthetic pathway in cabbage. In total, 62 CBGs were identified in the cabbage genome, which are orthologs of 47 CBGs in Arabidopsis thaliana. Out of the 62 CBGs, 46 genes in cabbage were mapped to nine chromosomes. Evolutionary analysis of carotenoid biosynthetic orthologous gene pairs among B. oleracea, B. rapa, and A. thaliana revealed that orthologous genes of B. oleracea underwent a negative selection similar to that of B. rapa. Expression analysis of the CBGs showed functional differentiation of orthologous gene copies in B. oleracea and B. rapa. Exogenous phytohormone treatment suggested that ETH, ABA, and MeJA can promote some important CBGs expression in cabbage. Phylogenetic analysis showed that BoPSYs exhibit high conservatism. Subcellular localization analysis indicated that BoPSYs are located in the chloroplast. This study is the first to study carotenoid biosynthesis genes in cabbage and provides a basis for further research on carotenoid metabolic mechanisms in cabbage.
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DOI：
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发表时间：
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