Ovarian stanniocalcin is structurally unique in mammals and its production and release are regulated through the luteinizing hormone receptor.
Ovarian stanniocalcin is structurally unique in mammals and its production and release are regulated through the luteinizing hormone receptor.
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卵巢斯钙素在哺乳动物中结构独特，其产生和释放通过黄体生成素受体调节。
作者：
M. Paciga;A. J. Watson;G. DiMattia;G. F. Wagner
斯钙素（Stanniocalcin，STC）是近年来发现的一种广泛分布于多种组织的哺乳动物激素。在啮齿类动物中，STC基因在卵巢中表达最高，特别是在产生雄激素的卵泡膜细胞和间质细胞中。此外，卵巢表达水平在怀孕期间上升15倍。本研究的目的是建立一个卵巢卵泡膜间质细胞（TIC）的原代培养系统，以确定STC的生产和释放的影响因素。我们使用了高度纯化的大鼠和牛TIC的原代培养物，其纯度常规评估抗原和酶标记。细胞的功能性通过它们对以孕酮释放形式的LH的反应来保证。我们发现，毛喉素显着增加STC基因的表达和分泌大鼠和牛的TIC，只有复制人（h）绒毛膜促性腺激素（CG）的效果。TIC与hCG和磷酸二酯酶抑制剂的共孵育进一步增加STC分泌，而TIC与hCG和蛋白激酶A抑制剂的共孵育减弱hCG刺激的释放。有趣的是，卵巢STC被证明比大多数其他组织中产生的50 kDa同源二聚体大得多。这些结果表明，卵巢STC是物理上不同的，一个功能，可以解释其存在于妊娠和哺乳期间的血清中。
Stanniocalcin (STC) is a recently discovered mammalian hormone that is widely distributed in many tissues. In rodents the STC gene is most highly expressed in ovary, specifically in androgen-producing thecal and interstitial cells. In addition, ovarian levels of expression rise 15-fold over pregnancy. The objective of this study was to develop a primary culture system for ovarian thecal-interstitial cells (TICs) to identify factors governing STC production and release. We used highly purified primary cultures of rat and bovine TICs, the purity of which was routinely assessed with antigenic and enzymatic markers. The functionality of cells was assured by their responsiveness to LH in the form of progesterone release. We found that forskolin significantly increased STC gene expression and secretion by both rat and bovine TICs, an effect that was only replicated by human (h) chorionic gonadotropin (CG). Coincubation of TICs with hCG and phosphodiesterase inhibitors further increased STC secretion, whereas coincubation of TICs with hCG and protein kinase A inhibitors attenuated hCG-stimulated release. Intriguingly, ovarian STC proved to be substantially larger than the 50-kDa homodimer produced in most other tissues. These results indicate that ovarian STC is physically distinct, a feature that could explain its presence in serum during pregnancy and lactation.
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大鼠卵巢胰岛素样生长因子结合蛋白-3：一种生长激素依赖性卵泡膜间质细胞衍生的抗促性腺激素。
影响因子：
4.8
作者：
Ricciarelli,E;Hernandez,ER;Tedeschi,C;Botero,LF;Kokia,E;Rohan,RM;Rosenfeld,RG;Albiston,AL;Herington,AC;Adashi,EY
通讯作者：
Adashi,EY
大鼠卵巢胰岛素样生长因子结合蛋白 6：一种激素调节的卵泡膜间质选择性物种，具有有限的抗促性腺激素活性。
影响因子：
4.8
作者：
Rohan,RM;Ricciarelli,E;Kiefer,MC;Resnick,CE;Adashi,EY
通讯作者：
Adashi,EY
低浓度和高浓度的促性腺激素不同地调节牛排卵前卵泡的内膜细胞和颗粒细胞产生的激素。
影响因子：
3.6
作者：
Berndtson,AK;Vincent,SE;Fortune,JE
通讯作者：
Fortune,JE
影响因子：
4.8
作者：
D. Magoffin;G. Erickson
通讯作者：
D. Magoffin;G. Erickson
DOI：
10.1016/s0021-9258(18)34752-5
发表时间：
1982-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. Magoffin;G. Erickson
通讯作者：
D. Magoffin;G. Erickson