A Comprehensive and Effective Mass Spectrometry-Based Screening Strategy for Discovery and Identification of New Brassinosteroids from Rice Tissues.
A Comprehensive and Effective Mass Spectrometry-Based Screening Strategy for Discovery and Identification of New Brassinosteroids from Rice Tissues.
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一种全面有效的基于质谱的筛选策略，用于从水稻组织中发现和鉴定新的油菜素类固醇
DOI：
10.3389/fpls.2016.01786
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发表时间：
2016
影响因子：
5.6
通讯作者：
Chu J
中科院分区：
文献类型：
--
作者：
Xin P;Yan J;Li B;Fang S;Fan J;Tian H;Shi Y;Tian W;Yan C;Chu J
新的油菜素内酯类化合物的发现和鉴定对于提高油菜素内酯类化合物的生物合成研究水平和扩大油菜素内酯类化合物的化学多样性具有重要意义。然而，传统的方法是劳动密集型的，耗时的，并且不太敏感。在这里，我们提出了一个简单的筛选策略，发现和鉴定新的BR植物组织的超高效液相色谱-质谱（UPLC-MS）的基础上。从仅1g水稻组织中发现了总共14个潜在的BR，并通过基于MS的线索进行结构解析，通过多反应监测（MRM）数据依赖的增强产物离子（EPI）扫描、高分辨率MS和MS调查依赖的MS/MS获得。14个候选物中的一个被鉴定为6-脱氧-28-高甾醇，在BR生物合成途径中首次报道的全新BR化合物。与参考标准的详细比较和水稻BR突变体的定量水平分析证实了其他候选物的可用性。这种有效而简单的方法为在植物中发现越来越多的BR生物合成中间体提供了一种有效的途径，这对于补充BR的生物合成和代谢网络具有重要意义。这种策略也可用于发现其他植物激素种类的未知化合物以及它们的关键代谢产物。
The exploration and identification of new brassinosteroid (BR) compounds is critical to improve the biosynthetic research of BRs and expand the chemodiversity of active BRs. However, traditional methods are labor-intensive, time-consuming, and less sensitive. Here, we present a facile screening strategy for discovering and identifying novel BRs from plant tissues based on ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). A total of 14 potential BRs were discovered from only 1 g of rice tissues and structurally elucidated by following a MS-based clue, acquired through multiple reaction monitoring (MRM) data-dependent enhanced product ion (EPI) scan, high resolution MS, and MS survey-dependent MS/MS. One of the 14 candidates was identified as 6-deoxo-28-homotyphasterol, a brand new BR compound that is reported for the first time in the BRs biosynthesis pathway. Detailed comparison with reference standards and quantitative level analysis in rice BR mutants confirmed the availability of the other candidates. This effective, yet simple method provides an efficient way to find more and more chemically new BR biosynthetic intermediates in plants, which is significant for complementing the biosynthesis and metabolism network of BRs. This strategy may also be used to discover unknown compounds of other plant hormone species as well as their key metabolites.
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影响因子：
5.1
作者：
Wan, Shuyan;Wu, Jinxia;Lu, Tiegang
通讯作者：
Lu, Tiegang
硼酸亲和力在通过 UPLC-MS/MS 高灵敏度检测亚克植物组织中的邻二醇油菜素类固醇中的双重作用
影响因子：
4.2
作者：
Xin, Peiyong;Yan, Jijun;Yan, Cunyu
通讯作者：
Yan, Cunyu
DOI：
10.1007/978-94-007-0189-2_12
发表时间：
2011-01-01
期刊：
BRASSINOSTEROIDS: A CLASS OF PLANT HORMONE
影响因子：
--
作者：
Hayat, Shamsul;Irfan, Mohammad;Ahmad, Aqil
通讯作者：
Ahmad, Aqil
影响因子：
1.6
作者：
FUJIOKA, S;INOUE, T;SAKURAI, A
通讯作者：
SAKURAI, A
影响因子：
2.9
作者：
Hwang, Jung Yun;Park, Chan Ho;Kim, Seong Ki
通讯作者：
Kim, Seong Ki