Translating metabolic exchange with imaging mass spectrometry.

Translating metabolic exchange with imaging mass spectrometry.
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DOI:
10.1038/nchembio.252
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发表时间:
2009-12
影响因子:
14.8
通讯作者:
Dorrestein PC
Dorrestein PC
中科院分区:
生物学1区
文献类型:
--
作者:
Yang YL;Xu Y;Straight P;Dorrestein PC

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生物体与环境之间的代谢交换,包括与邻近生物体的相互作用,对生物体发育过程很重要。在这里,我们开发和使用薄层琼脂天然产物MALDI-TOF成像质谱法的MALDI靶板上生长的完整的细菌菌落研究两种细菌之间的相互作用,并提供直接的证据,枯草芽孢杆菌沉默的防御武库天蓝色链霉菌。
Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide direct evidence that a Bacillus subtilis silences the defensive arsenal of Streptomyces coelicolor.
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