Partial resistance of SARS-CoV-2 Delta variants to vaccine-elicited antibodies and convalescent sera.
Partial resistance of SARS-CoV-2 Delta variants to vaccine-elicited antibodies and convalescent sera.
复制标题
SARS-COV-2 DELTA变体对疫苗吸收抗体和康复血清的部分抗性。
DOI：
10.1016/j.isci.2021.103341
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发表时间：
2021-11-19
期刊：
影响因子：
5.8
通讯作者：
Landau NR
中科院分区：
文献类型：
--
作者：
Tada T;Zhou H;Dcosta BM;Samanovic MI;Mulligan MJ;Landau NR
在印度发现的高度可传播的SARS-CoV-2变异株命名为B.1.617、Kappa(B.1.617.1)、Delta(B.1.617.2)、B.1.618和B.1.36.29，它们包含位于尖峰受体结合域内的尖峰突变L452R、T478K、E484K、E484Q和N440K，因此可能有助于增加传播性，并可能导致再次感染或对疫苗引发的抗体产生抗药性。为了解决这些问题，我们使用由变异棘突假型的慢病毒来测量恢复期血清、疫苗引发和再生治疗性抗体以及ACE2亲和力对它们的中和作用。恢复期血清和疫苗诱导的抗体中和了Delta尖峰病毒，不同捐赠者的滴度下降了2-5倍。Regeneron抗体鸡尾酒与Delta尖峰中和病毒，滴度下降2.6倍。L452R突变介导了对血清抗体和单抗的中和抗性。具有变异尖峰蛋白的病毒的抗体中和滴度的这些相对温和的下降表明，目前的疫苗仍将对Delta变种家族具有保护作用。疫苗诱导的抗体中和Delta Spike，滴度下降4-5倍Delta变体对REGN10933单抗具有抗药性中和耐药性由L452R生物科学介导；免疫反应；病毒学
Highly transmissible SARS-CoV-2 variants identified in India and designated B.1.617, Kappa (B.1.617.1), Delta (B.1.617.2), B.1.618, and B.1.36.29 contain spike mutations L452R, T478K, E484K, E484Q, and N440K located within the spike receptor-binding domain and thus could contribute to increased transmissibility and potentially allow re-infection or cause resistance to vaccine-elicited antibody. To address these issues, we used lentiviruses pseudotyped by variant spikes to measure their neutralization by convalescent sera, vaccine-elicited and Regeneron therapeutic antibodies, and ACE2 affinity. Convalescent sera and vaccine-elicited antibodies neutralized viruses with Delta spike with 2- to 5-fold decrease in titer in different donors. Regeneron antibody cocktail neutralized virus with the Delta spike with a 2.6-fold decrease in titer. Neutralization resistance to serum antibodies and monoclonal antibodies was mediated by L452R mutation. These relatively modest decreases in antibody neutralization titer for viruses with variant spike proteins suggest that current vaccines will remain protective against the family of Delta variants. Vaccine-elicited antibodies neutralize Delta spike with 4- to 5-fold decrease in titer Delta variant is resistant to REGN10933 monoclonal antibody Neutralization resistance is mediated by L452R Biological sciences; Immune response; Virology
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DOI：
10.1126/science.abc4730
发表时间：
2020-09-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gu H;Chen Q;Yang G;He L;Fan H;Deng YQ;Wang Y;Teng Y;Zhao Z;Cui Y;Li Y;Li XF;Li J;Zhang NN;Yang X;Chen S;Guo Y;Zhao G;Wang X;Luo DY;Wang H;Yang X;Li Y;Han G;He Y;Zhou X;Geng S;Sheng X;Jiang S;Sun S;Qin CF;Zhou Y
通讯作者：
Zhou Y
影响因子：
4.6
作者：
Noval MG;Kaczmarek ME;Koide A;Rodriguez-Rodriguez BA;Louie P;Tada T;Hattori T;Panchenko T;Romero LA;Teng KW;Bazley A;de Vries M;Samanovic MI;Weiser JN;Aifantis I;Cangiarella J;Mulligan MJ;Desvignes L;Dittmann M;Landau NR;Aguero-Rosenfeld M;Koide S;Stapleford KA
通讯作者：
Stapleford KA
影响因子：
82.9
作者：
Khoury, David S.;Cromer, Deborah;Davenport, Miles P.
通讯作者：
Davenport, Miles P.
影响因子：
64.5
作者：
Garcia-Beltran WF;Lam EC;St Denis K;Nitido AD;Garcia ZH;Hauser BM;Feldman J;Pavlovic MN;Gregory DJ;Poznansky MC;Sigal A;Schmidt AG;Iafrate AJ;Naranbhai V;Balazs AB
通讯作者：
Balazs AB
影响因子：
82.9
作者：
Xie, Xuping;Liu, Yang;Shi, Pei-Yong
通讯作者：
Shi, Pei-Yong