Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities.
Antibody isotype diversity against SARS-CoV-2 is associated with differential serum neutralization capacities.
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DOI：
10.1038/s41598-021-84913-3
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发表时间：
2021-03-10
影响因子：
4.6
通讯作者：
Stapleford KA
中科院分区：
文献类型：
--
作者：
Noval MG;Kaczmarek ME;Koide A;Rodriguez-Rodriguez BA;Louie P;Tada T;Hattori T;Panchenko T;Romero LA;Teng KW;Bazley A;de Vries M;Samanovic MI;Weiser JN;Aifantis I;Cangiarella J;Mulligan MJ;Desvignes L;Dittmann M;Landau NR;Aguero-Rosenfeld M;Koide S;Stapleford KA
了解对SARS-CoV-2的抗体反应对于制定遏制措施以克服当前的新冠肺炎大流行是不可或缺的。最近的研究表明，恢复期患者的血清可以表现出不同的中和能力。尽管如此，目前仍不清楚是否有特定的信号可以用来预测中和。在这里，我们对101名康复医护人员的血清进行了详细的分析，并用抗SARS-CoV-2刺突受体结合域和核蛋白的ELISA法研究了他们的SARS-CoV-2抗体反应。两种酶联免疫吸附试验方法均可检测血清中抗这两种抗原的持续水平。然而，我们队列中的大多数人产生了低中和能力的抗体，只有6%的人对真正的SARS-CoV-2病毒和Spike假型病毒表现出高中和效价。有趣的是，较高的中和血清与检测到抗这两种抗原的-Ig G、Ig M和Ig A抗体相关，而仅有Ig G阳性的个体表现出较差的中和反应。这些结果表明，拥有更广泛的抗体库可能有助于更有效地中和SARS-CoV-2。总之，我们的工作提供了康复医护人员SARS-CoV-2中和抗体应答的横断面快照，并提供了初步证据，表明拥有多种抗体亚型可以在预测SARS-CoV-2中和作用方面发挥重要作用。
Understanding antibody responses to SARS-CoV-2 is indispensable for the development of containment measures to overcome the current COVID-19 pandemic. Recent studies showed that serum from convalescent patients can display variable neutralization capacities. Still, it remains unclear whether there are specific signatures that can be used to predict neutralization. Here, we performed a detailed analysis of sera from a cohort of 101 recovered healthcare workers and we addressed their SARS-CoV-2 antibody response by ELISA against SARS-CoV-2 Spike receptor binding domain and nucleoprotein. Both ELISA methods detected sustained levels of serum IgG against both antigens. Yet, the majority of individuals from our cohort generated antibodies with low neutralization capacity and only 6% showed high neutralizing titers against both authentic SARS-CoV-2 virus and the Spike pseudotyped virus. Interestingly, higher neutralizing sera correlate with detection of -IgG, IgM and IgA antibodies against both antigens, while individuals with positive IgG alone showed poor neutralization response. These results suggest that having a broader repertoire of antibodies may contribute to more potent SARS-CoV-2 neutralization. Altogether, our work provides a cross sectional snapshot of the SARS-CoV-2 neutralizing antibody response in recovered healthcare workers and provides preliminary evidence that possessing multiple antibody isotypes can play an important role in predicting SARS-CoV-2 neutralization.
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影响因子：
15.3
作者：
Schmidt, Fabian;Weisblum, Yiska;Bieniasz, Paul D.
通讯作者：
Bieniasz, Paul D.
影响因子：
24.8
作者：
Isho B;Abe KT;Zuo M;Jamal AJ;Rathod B;Wang JH;Li Z;Chao G;Rojas OL;Bang YM;Pu A;Christie-Holmes N;Gervais C;Ceccarelli D;Samavarchi-Tehrani P;Guvenc F;Budylowski P;Li A;Paterson A;Yue FY;Marin LM;Caldwell L;Wrana JL;Colwill K;Sicheri F;Mubareka S;Gray-Owen SD;Drews SJ;Siqueira WL;Barrios-Rodiles M;Ostrowski M;Rini JM;Durocher Y;McGeer AJ;Gommerman JL;Gingras AC
通讯作者：
Gingras AC
影响因子：
82.9
作者：
Amanat F;Stadlbauer D;Strohmeier S;Nguyen THO;Chromikova V;McMahon M;Jiang K;Arunkumar GA;Jurczyszak D;Polanco J;Bermudez-Gonzalez M;Kleiner G;Aydillo T;Miorin L;Fierer DS;Lugo LA;Kojic EM;Stoever J;Liu STH;Cunningham-Rundles C;Felgner PL;Moran T;García-Sastre A;Caplivski D;Cheng AC;Kedzierska K;Vapalahti O;Hepojoki JM;Simon V;Krammer F
通讯作者：
Krammer F
影响因子：
32.4
作者：
Schultheiss, Christoph;Paschold, Lisa;Binder, Mascha
通讯作者：
Binder, Mascha
DOI：
10.1016/s0140-6736(20)31611-1
发表时间：
2020-08-15
期刊：
Lancet (London, England)
影响因子：
--
作者：
Bar-Zeev N;Moss WJ
通讯作者：
Moss WJ