Three-dimensional distribution of cortical synapses: a replicated point pattern-based analysis.
Three-dimensional distribution of cortical synapses: a replicated point pattern-based analysis.
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DOI：
10.3389/fnana.2014.00085
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发表时间：
2014
影响因子：
2.9
通讯作者：
Larrañaga P
中科院分区：
文献类型：
--
作者：
Anton-Sanchez L;Bielza C;Merchán-Pérez A;Rodríguez JR;DeFelipe J;Larrañaga P
分析大脑时最大的问题是它的突触连接极其复杂。一般来说，组成大脑的数十亿个神经元通过两种高度专业化的结构交换信息：化学突触(绝大多数)和所谓的缝隙连接(一类电子突触的底物)。在这里，我们感兴趣的是探索大脑皮层化学突触的三维空间分布。最近的研究表明，突触在大脑皮层第三层的三维空间分布可以用随机顺序吸附(RSA)点过程来模拟，即突触在空间上几乎是随机分布的，唯一的约束是它们不能重叠。在这项研究中，我们假设RSA过程也可以解释突触在所有皮质层的分布。我们还研究了突触密度和突触在各层之间的空间分布是否存在差异。利用聚焦离子束研磨和扫描电子显微镜(FIB/SEM)相结合的方法，从大鼠体感觉皮质的6层获得三维标本，并对突触连接进行识别和重建。分析了来自三种不同动物的大约4500μ立方米的组织和大约4000个突触的总体积。不同的样本、蛋鸡和/或动物被聚集，并使用RSA复制的空间点过程进行比较。结果表明，在研究中使用的不同大鼠之间的突触分布没有显著差异。我们发现RSA过程描述了突触在每一层的所有样本中的空间分布。我们还发现，突触在第II至第VI层的分布符合一个共同的潜在RSA过程，每层的密度不同。有趣的是，结果显示，第I层的突触与其他层的突触的空间分布略有不同。
The biggest problem when analyzing the brain is that its synaptic connections are extremely complex. Generally, the billions of neurons making up the brain exchange information through two types of highly specialized structures: chemical synapses (the vast majority) and so-called gap junctions (a substrate of one class of electrical synapse). Here we are interested in exploring the three-dimensional spatial distribution of chemical synapses in the cerebral cortex. Recent research has showed that the three-dimensional spatial distribution of synapses in layer III of the neocortex can be modeled by a random sequential adsorption (RSA) point process, i.e., synapses are distributed in space almost randomly, with the only constraint that they cannot overlap. In this study we hypothesize that RSA processes can also explain the distribution of synapses in all cortical layers. We also investigate whether there are differences in both the synaptic density and spatial distribution of synapses between layers. Using combined focused ion beam milling and scanning electron microscopy (FIB/SEM), we obtained three-dimensional samples from the six layers of the rat somatosensory cortex and identified and reconstructed the synaptic junctions. A total volume of tissue of approximately 4500μm3 and around 4000 synapses from three different animals were analyzed. Different samples, layers and/or animals were aggregated and compared using RSA replicated spatial point processes. The results showed no significant differences in the synaptic distribution across the different rats used in the study. We found that RSA processes described the spatial distribution of synapses in all samples of each layer. We also found that the synaptic distribution in layers II to VI conforms to a common underlying RSA process with different densities per layer. Interestingly, the results showed that synapses in layer I had a slightly different spatial distribution from the other layers.
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影响因子：
64.8
作者：
Bock, Davi D.;Lee, Wei-Chung Allen;Kerlin, Aaron M.;Andermann, Mark L.;Hood, Greg;Wetzel, Arthur W.;Yurgenson, Sergey;Soucy, Edward R.;Kim, Hyon Suk;Reid, R. Clay
通讯作者：
Reid, R. Clay
影响因子：
5.8
作者：
Baddeley, A;Turner, R
通讯作者：
Turner, R
影响因子：
3.7
作者：
Burguet J;Andrey P
通讯作者：
Andrey P
影响因子：
6.1
作者：
Baddeley, Adrian;Diggle, Peter J.;Nair, Gopalan
通讯作者：
Nair, Gopalan
DOI：
10.1073/pnas.0803652105
发表时间：
2008-09-23
影响因子：
11.1
作者：
Alonso-Nanclares, L.;Gonzalez-Soriano, J.;DeFelipe, J.
通讯作者：
DeFelipe, J.