Through the looking glass: A review of cranial window technology for optical access to the brain.

Through the looking glass: A review of cranial window technology for optical access to the brain.
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透过镜子:一个审查颅窗技术的光学访问大脑。

DOI:
10.1016/j.jneumeth.2021.109100
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发表时间:
2021-04-15
影响因子:
3
通讯作者:
Chen CC
Chen CC
中科院分区:
医学4区
文献类型:
--
作者:
Cramer SW;Carter RE;Aronson JD;Kodandaramaiah SB;Ebner TJ;Chen CC

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破译神经功能是一项艰巨的任务,需要了解神经元网络和单个神经元之间相互作用产生的新特性。对神经元网络的机械洞察需要同时评估单个神经元活动和随之而来的中尺度输出的工具。颅窗技术的发展,将头骨变薄或用合成的光学界面取代,使监测清醒的动物的神经元活动从亚细胞到中尺度分辨率成为可能,当与先进的显微镜技术相结合时。本文回顾了近年来颅窗技术的研究成果,评价了各种设计方案的优劣,并讨论了今后颅窗设计的研究方向。
Deciphering neurologic function is a daunting task, requiring understanding the neuronal networks and emergent properties that arise from the interactions among single neurons. Mechanistic insights into neuronal networks require tools that simultaneously assess both single neuron activity and the consequent mesoscale output. The development of cranial window technologies, in which the skull is thinned or replaced with a synthetic optical interface, has enabled monitoring neuronal activity from subcellular to mesoscale resolution in awake, behaving animals when coupled with advanced microscopy techniques. Here we review recent achievements in cranial window technologies, appraise the relative merits of each design and discuss the future research in cranial window design.
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