Characterization of regulatory regions involved in the inducible expression of chiB in Bacillus thuringiensis
Characterization of regulatory regions involved in the inducible expression of chiB in Bacillus thuringiensis
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苏云金芽孢杆菌 chiB 诱导表达调控区的表征
DOI：
10.1007/s00203-014-1054-3
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Yue‑Hua Chen
中科院分区：
文献类型：
--
作者：
Xie Chi‑Chu;Jin Shi;Hai‑Yun Jia;Peng‑Fei Li;Yang Luo;Jun Cai;Yue‑Hua Chen
Expression of the chiB gene from Bacillus thuringiensis Bti75 was defined as inducible by the use of transcriptional fusions with the bgaB reporter gene. The transcription start site of the chiB gene was identified as the C base located 132 base pairs upstream of the start codon. Analysis of 5' and 3' deletions of the chiB promoter region revealed that the sequence from position -192 to +36 with respect to the transcription start site was necessary for wild-type levels of inducible expression of the chiB gene. The minimal promoter region for the expression of chiB gene was identified as the sequence from position -100 to +12. Furthermore, a 16-bp sequence (designated dre) downstream of the minimal promoter region of chiB was shown to be required for chitin induction. To confirm the function of this 16-bp sequence, 25 base substitutions were introduced into the dre site. Most of the mutations resulted in constitutive expression, or the efficiency of induction decreased. All mutations identified the dre sequence as a critical site for the inducible expression of chiB. In addition, the dre site was shown to interact with a sequence-specific DNA binding factor of strain Bti75 cultured in the absence of the inducer.
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影响因子：
2.8
作者：
Shu-yi Wang;Shaw-Jye Wu;G. Thottappilly;R. Locy;N. Singh
通讯作者：
Shu-yi Wang;Shaw-Jye Wu;G. Thottappilly;R. Locy;N. Singh
影响因子：
4.7
作者：
Chandrasekaran, Rajamanickam;Revathi, Kannan;Senthil-Nathan, Sengottayan
通讯作者：
Senthil-Nathan, Sengottayan
影响因子：
3.2
作者：
Purushotham, Pallinti;Podile, Appa Rao
通讯作者：
Podile, Appa Rao
DOI：
10.1111/j.1432-1033.1985.tb09049.x
发表时间：
1985-08
期刊：
European journal of biochemistry
影响因子：
--
作者：
M. Kimura;J. Kimura;K. Ashman
通讯作者：
M. Kimura;J. Kimura;K. Ashman
DOI：
10.1073/pnas.89.5.1885
发表时间：
1992-03-01
影响因子：
11.1
作者：
DELIC, I;ROBBINS, P;WESTPHELING, J
通讯作者：
WESTPHELING, J