Measles vaccination using a microneedle patch.
Measles vaccination using a microneedle patch.
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DOI：
10.1016/j.vaccine.2012.09.062
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发表时间：
2013-07-25
期刊：
影响因子：
5.5
通讯作者：
Prausnitz, Mark R.
中科院分区：
文献类型：
--
作者：
Edens, Chris;Collins, Marcus L.;Ayers, Jessica;Rota, Paul A.;Prausnitz, Mark R.
麻疹疫苗接种计划将受益于降低成本、简化物流和提高安全性的交付方法。传统的皮下注射受到以下因素的限制：需要熟练的医疗保健专业人员来恢复和管理注射，以及需要安全的针头处理和处置，以减少通过针头重复使用和针头损伤而传播疾病的风险。微针是一种微米级的实心针，上面覆盖着一种干燥的疫苗配方，贴片后几分钟内就会在皮肤中溶解。通过避免使用皮下注射针，使用微针贴片的疫苗接种可以由受过最低限度培训的人员进行，从而降低血液传播疾病的风险。这项研究的目的是评估使用微针贴片接种麻疹疫苗，以解决皮下注射的一些局限性。在微针贴片上干燥的疫苗病毒通过加入糖和海藻糖来稳定活性，在室温下储存至少30天后，病毒滴度损失小于1log10(TCID50)。然后用微针贴片用埃德蒙斯顿-萨格勒布麻疹疫苗株免疫棉鼠。使用微针接种的剂量等于标准人体剂量或人体剂量的五分之一，产生的中和抗体水平相当于相同剂量的皮下免疫。这些结果表明，麻疹疫苗可以稳定在微针上，疫苗在体内有效地重组，产生与皮下注射相当的中和抗体应答。
Measles vaccination programs would benefit from delivery methods that decrease cost, simplify logistics, and increase safety. Conventional subcutaneous injection is limited by the need for skilled healthcare professionals to reconstitute and administer injections, and by the need for safe needle handling and disposal to reduce the risk of disease transmission through needle re-use and needlestick injury. Microneedles are micron-scale, solid needles coated with a dry formulation of vaccine that dissolves in the skin within minutes after patch application. By avoiding the use of hypodermic needles, vaccination using a microneedle patch could be carried out by minimally trained personnel with reduced risk of blood-borne disease transmission. The goal of this study was to evaluate measles vaccination using a microneedle patch to address some of the limitations of subcutaneous injection. Viability of vaccine virus dried onto a microneedle patch was stabilized by incorporation of the sugar, trehalose, and loss of viral titer was less than 1 log10(TCID50) after storage for at least 30 days at room temperature. Microneedle patches were then used to immunize cotton rats with the Edmonston-Zagreb measles vaccine strain. Vaccination using microneedles at doses equaling the standard human dose or one-fifth the human dose generated neutralizing antibody levels equivalent to those of a subcutaneous immunization at the same dose. These results show that measles vaccine can be stabilized on microneedles and that vaccine efficiently reconstitutes in vivo to generate a neutralizing antibody response equivalent to that generated by subcutaneous injection.
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DOI：
10.1086/649228
发表时间：
2010-01-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Kim YC;Quan FS;Yoo DG;Compans RW;Kang SM;Prausnitz MR
通讯作者：
Prausnitz MR
影响因子：
1.7
作者：
Cutts, FT;Clements, CJ;Bennett, JV
通讯作者：
Bennett, JV
影响因子：
3.7
作者：
Daddona, Peter E.;Matriano, James. A.;Maa, Yuh-Fun
通讯作者：
Maa, Yuh-Fun
影响因子：
3.7
作者：
Fernando GJ;Chen X;Prow TW;Crichton ML;Fairmaid EJ;Roberts MS;Frazer IH;Brown LE;Kendall MA
通讯作者：
Kendall MA
影响因子：
3.7
作者：
Corbett HJ;Fernando GJ;Chen X;Frazer IH;Kendall MA
通讯作者：
Kendall MA