CD44 promotes cell proliferation in non-small cell lung cancer.
CD44 promotes cell proliferation in non-small cell lung cancer.
复制标题
CD44促进非小细胞肺癌细胞增殖
DOI：
10.3892/ol.2018.8051
复制
发表时间：
2018-04
期刊：
影响因子：
2.9
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Hu B;Ma Y;Yang Y;Zhang L;Han H;Chen J
据报道，分化簇 44 (CD44) 是癌症干细胞的标志物，与非小细胞肺癌 (NSCLC) 的不良预后相关，但其与肿瘤生长的关系尚未完全阐明。本研究利用免疫组织化学方法探讨了 94 例 NSCLC 病例中 CD44 表达与临床病理特征之间的关联，以及该蛋白异常表达的细胞增殖情况。通过用CD44表达载体转染H1299细胞实现CD44的过表达，并通过将小干扰RNA转染到A549细胞中来抑制CD44表达。使用 Cell Counting Kit-8 测定法测量细胞增殖，并进行平板集落形成测定法以确认体外细胞贴壁生长。总共，94 例 NSCLC 病例中有 64 例 (68.1%) CD44 表达呈阳性。 CD44 的高表达与 NSCLC 的晚期 T 期相关。 H1299细胞中CD44的过度表达促进细胞增殖和体外集落形成。同时，A549细胞中CD44表达的敲低抑制了体外细胞增殖和集落形成。 CD44的高表达可能通过增加癌细胞增殖来促进NSCLC进展；因此，它可以作为诊断或靶向治疗的潜在生物标志物。
Cluster of differentiation 44 (CD44), a marker for cancer stem cells, has been reported to be associated with poor prognosis in non-small cell lung cancer (NSCLC), but its involvement in tumor growth has not fully been elucidated. The present study explored the associations between CD44 expression and clinicopathological features using immunohistochemistry in 94 NSCLC cases, as well as proliferation in cells with aberrant expression of this protein. Overexpression of CD44 was achieved by transfecting H1299 cells with CD44 expression vectors, and inhibition of CD44 expression was performed by transfecting small interfering RNAs into A549 cells. Cell proliferation was measured using Cell Counting Kit-8 assays and flat plate colony formation assay was performed to confirm the cellular anchorage growth in vitro. In total, 64 (68.1%) of the 94 NSCLC cases stained positive for CD44 expression. High expression of CD44 was associated with advanced T stage in NSCLC. Overexpression of CD44 in H1299 cells promoted cell proliferation and colony formation in vitro. Meanwhile, knockdown of CD44 expression in A549 cells suppressed cell proliferation and colony formation in vitro. High expression of CD44 may promote NSCLC progression by increasing cancer cell proliferation; therefore, it may serve as a potential biomarker for diagnosis or target therapy.
登录
查看更多内容
CD44 剪接变体 v8-10 作为浆液性卵巢癌预后的标志。
影响因子：
3.7
作者：
Sosulski A;Horn H;Zhang L;Coletti C;Vathipadiekal V;Castro CM;Birrer MJ;Nagano O;Saya H;Lage K;Donahoe PK;Pépin D
通讯作者：
Pépin D
影响因子：
3.7
作者：
Aso, Takeichiro;Matsuo, Mioko;Masuda, Muneyuki
通讯作者：
Masuda, Muneyuki
CD44 在疾病病理生理学和靶向治疗中的作用。
影响因子：
7.3
作者：
Jordan AR;Racine RR;Hennig MJ;Lokeshwar VB
通讯作者：
Lokeshwar VB
表达 CD44 的非小细胞肺癌细胞富含干细胞样特性。
影响因子：
3.7
作者：
Leung, Elaine Lai-Han;Fiscus, Ronald R.;Tung, James W.;Tin, Vicky Pui-Chi;Cheng, Lik Cheung;Sihoe, Alan Dart-Loon;Fink, Louis M.;Ma, Yupo;Wong, Maria Pik
通讯作者：
Wong, Maria Pik
影响因子：
3.8
作者：
Chen J;Xie F;Zhang L;Jiang WG
通讯作者：
Jiang WG