H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination.
H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination.
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DOI：
10.1093/jxb/erq125
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发表时间：
2010-06
影响因子：
6.9
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Liu Y;Ye N;Liu R;Chen M;Zhang J
H_2O_2是植物细胞中的信号分子，但其在调节阿坝和GA代谢及激素平衡中的作用尚不清楚。本研究发现，H_2O_2影响种子吸胀过程中阿坝催化和GA生物合成的调节，从而控制种子休眠和萌发。定量RT-PCR（QRT-PCR）结果表明，H2 O2上调了阿坝催化基因（如CYP 707 A基因），导致吸胀过程中阿坝含量下降。这一作用需要另一种信号分子一氧化氮（NO）的参与。同时，QRT-PCR结果表明，H2 O2也能促进GA的合成。当阿坝catalysts突变体，CYP 707 A2，和过表达的植物，CYP 707 A2-OE，进行了测试，阿坝含量与GA生物合成呈负相关。外源GA能克服低浓度阿坝对种子萌发的抑制作用，但对高浓度阿坝的抑制作用不明显。H_2O_2可能通过NO信号介导阿坝催化剂的上调，并促进GA的合成。高浓度的阿坝抑制GA的生物合成，但这两种激素的平衡可以共同控制拟南芥种子的休眠和萌发。
H2O2 is known as a signal molecule in plant cells, but its role in the regulation of aqbscisic acid (ABA) and gibberellic acid (GA) metabolism and hormonal balance is not yet clear. In this study it was found that H2O2 affected the regulation of ABA catabolism and GA biosynthesis during seed imbibition and thus exerted control over seed dormancy and germination. As seen by quantitative RT-PCR (QRT-PCR), H2O2 up-regulated ABA catabolism genes (e.g. CYP707A genes), resulting in a decreased ABA content during imbibition. This action required the participation of nitric oxide (NO), another signal molecule. At the same time, H2O2 also up-regulated GA biosynthesis, as shown by QRT-PCR. When an ABA catabolism mutant, cyp707a2, and an overexpressing plant, CYP707A2-OE, were tested, ABA content was negatively correlated with GA biosynthesis. Exogenously applied GA was able to over-ride the inhibition of germination at low concentrations of ABA, but had no obvious effect when ABA concentrations were high. It is concluded that H2O2 mediates the up-regulation of ABA catabolism, probably through an NO signal, and also promotes GA biosynthesis. High concentrations of ABA inhibit GA biosynthesis but a balance of these two hormones can jointly control the dormancy and germination of Arabidopsis seeds.
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