Transcriptome analysis reveals a regulation of ethylene-induced post-harvest senescence in pear fruit

Transcriptome analysis reveals a regulation of ethylene-induced post-harvest senescence in pear fruit
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转录组分析揭示了乙烯诱导的梨果实采后衰老的调节

DOI:
10.1016/j.scienta.2018.05.019
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发表时间:
2018-10
影响因子:
4.3
通讯作者:
Zhang SL
Zhang SL
中科院分区:
农林科学2区
文献类型:
--
作者:
Xu Huan-Yu;Chen Yang-Yang;Wang Li-Bin;Xie Zhi-Hua;Gu Chao;Zhang Shao-Ling;Gu C;Zhang SL

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乙烯可诱导梨果实衰老,但具体是哪些乙烯反应因子参与其中尚不清楚。在这项研究中,成熟的果实cv。分别用乙烯利和1-MCP处理厚穗果实,对处理后的果实进行转录组测序,以揭示乙烯诱导衰老相关基因。结果表明,乙烯生物合成和信号通路基因在腐烂前果实中的表达量明显高于成熟期果实,但在采后果实中只有一个新的ERF基因(Pbr 022708.1)在贮藏过程中受到乙烯的诱导。此外,基于果实硬度的变化,乙烯诱导的基因,分别编码多聚半乳糖醛酸酶(Pbr 010853.1)和木葡聚糖内切转葡糖基酶/水解酶(Pbr 040203.1)被分离到与果实采后贮藏软化。此外,生长素信号和胁迫耐受性可能参与果实衰老。这些结果将有助于了解采后果实贮藏过程中的基因调控。
Fruit senescence is induced by ethylene in pear, but it is unclear which ethylene responsive factor(s) is involved in. In this study, the ripening fruits of cv. Housui were respectively treated by ethephon and 1-MCP, and the treated fruits were used for transcriptome sequencing to reveal the genes associated with ethylene-induced senescence. As expected, ethylene biosynthesis and signal pathway genes were detected and had remarkably higher levels of expression in all the pre-decayed fruits than in the ripening fruits, but only one novelERFgene (Pbr022708.1) was induced by ethylene in post-harvested fruits during storage. Moreover, based on the changes of fruit firmness, two ethylene-induced genes that individually encode polygalacturonase (Pbr010853.1) and xyloglucan endotransglucosylase/hydrolase (Pbr040203.1) were isolated to be associated with fruit softening during post-harvest storage. In addition, auxin signal and stress tolerance were likely involved into fruit senescence. These result will be available for understanding gene regulation of post-harvested fruits during storage.
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