Characterization of a novel detergent-resistant type I pullulanase from Bacillus megaterium Y103 and its application in laundry detergent
Characterization of a novel detergent-resistant type I pullulanase from Bacillus megaterium Y103 and its application in laundry detergent
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巨大芽孢杆菌Y103新型耐洗涤剂I型支链淀粉酶的表征及其在洗衣粉中的应用
DOI：
10.1080/10826068.2022.2134890
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发表时间：
2022-10
影响因子：
2.9
通讯作者：
Yuchun Liu
中科院分区：
文献类型：
--
作者：
Yongmin Wu;Shuai Huang;Xiaobo Liang;Peng Han;Yuchun Liu
本研究旨在寻找一种适合洗涤剂工业的普鲁兰酶。从巨大芽孢杆菌Y103中克隆了pulY 103 B基因（2217 bp），并在大肠杆菌中表达。PulY 103 B含有糖苷水解酶家族（GH）13的4个保守区和I型普鲁兰酶的典型序列。PulY 103 B的最佳反应条件为pH6.5和40 °C。此外，它在40 °C以下保持稳定，并且在pH 6.0至8.5范围内保持超过80%的活性。该酶的最佳底物为普鲁兰多糖。对小麦淀粉（36.5%）和可溶性淀粉（33.4%）有活性，对直链淀粉和糖原无活性。麦芽三糖和麦芽六糖是普鲁兰多糖水解的主要产物。可溶性淀粉和支链淀粉主要水解为麦芽四糖。这些结果表明PulY 103 B是一种新型的I型普鲁兰酶，具有通过α-1，4-糖苷键的形成进行转糖基化的活性。此外，PulY 103 B被非离子洗涤剂[即，Tween 20（10%）、Tween 80（1%）、Triton X-100（20%）]和商业液体洗涤剂（3.0g/L）强烈刺激。洗涤性能测试表明，PulY 103 B和洗涤剂的混合物比单独使用洗涤剂更好地去除淀粉基污渍（p< 0.05）。因此，该普鲁兰酶作为洗涤剂添加剂具有很大的潜力。
This study aims to find a moderate pullulanase for detergent industry. ThepulY103Bgene (2217 bp) fromBacillus megateriumY103 was cloned and expressed inEscherichia coli. PulY103B contained four conserved regions of glycoside hydrolase family (GH) 13 and the typical sequence of type I pullulanase. The optimal reaction conditions of PulY103B were pH 6.5 and 40 °C. In addition, it remained stable below 40 °C and over 80% of activity was retained at pH ranging from 6.0 to 8.5. The best substrate for the enzyme was pullulan. Furthermore, it exhibited activity toward wheat starch (36.5%) and soluble starch (33.4%) but had no activity toward amylose and glycogen. Maltotriose and maltohexaose were major pullulan hydrolysis products. Soluble starch and amylopectin were mainly hydrolyzed into maltotetraose. These results indicated that PulY103B is a novel type I pullulanase with transglycosylation activity via formation of α-1,4-glucosidic linkages. Moreover, PulY103B was strongly stimulated by nonionic detergents [viz, Tween 20 (10%), Tween 80 (1%), Triton X-100 (20%)] and commercial liquid detergents (3.0 g/L). Wash performance tests demonstrated that the mixture of PulY103B and detergent removed starch-based stains better than using detergent alone (p< 0.05). Therefore, this pullulanase has big potential as a detergent additive.
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影响因子：
11.4
作者：
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通讯作者：
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影响因子：
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DOI：
10.1016/j.foodchem.2017.06.117
发表时间：
2018
期刊：
Food Chem
影响因子：
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作者：
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