In vitro three-dimensional organotypic culture models of the oral mucosa.
In vitro three-dimensional organotypic culture models of the oral mucosa.
复制标题
口腔粘膜体外三维器官型培养模型。
DOI：
10.1007/s11626-020-00539-1
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Aizawa S
中科院分区：
文献类型：
--
作者：
Klausner M;Handa Y;Aizawa S
已经开发了口腔粘膜的三维器官型模型来研究口腔中发生的各种各样的现象。虽然学术研究实验室已经开发了许多模型，但只有少数模型已经商业化。来自学术团体的模型提供了更广泛的表型，而商业模型更侧重于口腔和牙龈粘膜。商业化的模型是在高度受控的条件下生产的，符合质量标准的要求，这导致了高水平的再现性。这些体外模型已用于评价口腔护理产品（如牙膏、漱口水和粘膜粘合剂）的刺激性。研究了香烟烟雾对口腔组织的影响，并与电子烟进行了比较。口腔组织模型有助于研究口腔粘膜炎和口腔念珠菌病的机制，并已被用于检查经颊药物递送速率和纳米颗粒的吸收。感染研究已经调查了HIV-1沿着细菌和病原菌的影响。最近，分化的口腔组织模型已显示表达ACE2受体，已知ACE2受体对于SARS-CoV-2冠状病毒通过受体介导进入人体细胞和组织是重要的。因此，口腔粘膜模型可用于确定口腔粘膜是否可能发生病毒感染，以及这种感染是否对当前的COVID-19大流行有影响。显而易见，这些模型用于广泛的应用中，并且在可重复性、避免物种外推以及与人类和动物实验相关的伦理问题方面通常比动物模型具有优势。本文的目标是审查市售模型的人类颊和牙龈粘膜，并强调其使用，以获得更好地了解广泛的现象，影响口腔组织。
Three-dimensional, organotypic models of the oral mucosa have been developed to study a wide variety of phenomena occurring in the oral cavity. Although a number of models have been developed in academic research labs, only a few models have been commercialized. Models from academic groups offer a broader range of phenotypes while the commercial models are more focused on the oral and gingival mucosa. The commercialized models are manufactured under highly controlled conditions and meet the requirements of quality standards, which leads to high levels of reproducibility. These in vitro models have been used to evaluate the irritancy of oral care products such as toothpastes, mouthwashes, and mucoadhesives. The effects of cigarette smoke on oral cavity tissues have been studied and compared to those of e-cigarettes. Oral tissue models have facilitated investigation of the mechanisms of oral mucositis and oral candidiasis and have been used to examine transbuccal drug delivery rates and the absorption of nanoparticles. Infection studies have investigated the effects of HIV-1 along with the effects of commensal and pathogenic bacteria. More recently, a differentiated oral tissue model has been shown to express the ACE2 receptor, which is known to be important for the receptor-mediated entry of the SARS-CoV-2 coronavirus into human cells and tissues. Hence, oral mucosal models may find application in determining whether viral infection of the oral mucosa is possible and whether such infection has implications vis-a-vis the current COVID-19 pandemic. As is apparent, these models are used in a broad variety of applications and often offer advantages versus animal models in terms of reproducibility, avoiding species extrapolation, and the ethical concerns related to human and animal experimentation. The goals of this paper are to review commercially available models of the human buccal and gingival mucosa and highlight their use to gain a better understanding of a broad range of phenomena affecting tissues in the oral cavity.
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影响因子：
4.6
作者：
Hisha, Hiroko;Tanaka, Toshihiro;Kanno, Shohei;Tokuyama, Yoko;Komai, Yoshihiro;Ohe, Shuichi;Yanai, Hirotsugu;Omachi, Taichi;Ueno, Hiroo
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28.2
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5.4
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通讯作者：
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影响因子：
14.8
作者：
Dongari-Bagtzoglou, Anna;Kashleva, Helena
通讯作者：
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