Real-time evaluation of aggregation using confocal imaging and image analysis tools.

Real-time evaluation of aggregation using confocal imaging and image analysis tools.
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使用共焦成像和图像分析工具实时评估聚集。

DOI:
10.1039/c3an01693e
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发表时间:
2014
期刊:
The Analyst
影响因子:
--
通讯作者:
Hamrang Z
Hamrang Z
中科院分区:
--
文献类型:
--
作者:
Hamrang Z

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实时共聚焦成像被用来监测原位损失的BSA单体和聚集体的形成,使用空间强度分布分析(SpIDA)和光栅图像相关光谱(RICS)。在概念水平的证明,这项工作已经证明了RICS和SpIDA监测蛋白质寡聚化和更大的聚集体形成的适用性。
Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster Image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.
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