Limited systemic sclerosis patients with pulmonary arterial hypertension show biomarkers of inflammation and vascular injury.
Limited systemic sclerosis patients with pulmonary arterial hypertension show biomarkers of inflammation and vascular injury.
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DOI：
10.1371/journal.pone.0012106
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发表时间：
2010-08-17
期刊：
影响因子：
3.7
通讯作者：
Lafyatis R
中科院分区：
文献类型：
--
作者：
Pendergrass SA;Hayes E;Farina G;Lemaire R;Farber HW;Whitfield ML;Lafyatis R
肺动脉高压（PAH）是局限性系统性硬化症（lSSc）患者的常见并发症。lSSc-PAH生物标志物的鉴定和表征应导致侵入性更小的筛查，更好地理解发病机制和改善治疗。从21名无PAH的lSSc受试者（lSSc-noPAH）、15名患有PAH的lSSc受试者（lSSc-PAH）和10名健康对照者获得49份PBMC样品;三名受试者在一年后提供PBMC。测量每个样品的全基因组基因表达。通过多分析物分析（MAP）免疫测定法测量来自受试者子集的血清中89种细胞因子的水平。基因表达清楚地区分了lSSc样品与健康对照，并将lSSc-PAH与lSSc-NoPAH患者分开。实时定量PCR证实lSSc-PAH患者中9个基因（ICAM 1、IFNGR 1、IL 1B、IL 13 Ra 1、JAK 2、AIF 1、CCR 1、ALAS 2、TIMP 2）表达增加。在lSSc-PAH受试者中发现炎性介质（如TNF-α、IL 1-β、ICAM-1和IL-6）和血管损伤标志物（如VCAM-1、VEGF和血管性血友病因子）的循环细胞因子水平升高。lSSc-PAH患者的基因表达和细胞因子谱表明存在活化的单核细胞，并显示血管损伤和炎症的标志物。这些基因和因子可以作为PAH参与lSSc的生物标志物。
Pulmonary arterial hypertension (PAH) is a common complication for individuals with limited systemic sclerosis (lSSc). The identification and characterization of biomarkers for lSSc-PAH should lead to less invasive screening, a better understanding of pathogenesis, and improved treatment. Forty-nine PBMC samples were obtained from 21 lSSc subjects without PAH (lSSc-noPAH), 15 lSSc subjects with PAH (lSSc-PAH), and 10 healthy controls; three subjects provided PBMCs one year later. Genome-wide gene expression was measured for each sample. The levels of 89 cytokines were measured in serum from a subset of subjects by Multi-Analyte Profiling (MAP) immunoassays. Gene expression clearly distinguished lSSc samples from healthy controls, and separated lSSc-PAH from lSSc-NoPAH patients. Real-time quantitative PCR confirmed increased expression of 9 genes (ICAM1, IFNGR1, IL1B, IL13Ra1, JAK2, AIF1, CCR1, ALAS2, TIMP2) in lSSc-PAH patients. Increased circulating cytokine levels of inflammatory mediators such as TNF-alpha, IL1-beta, ICAM-1, and IL-6, and markers of vascular injury such as VCAM-1, VEGF, and von Willebrand Factor were found in lSSc-PAH subjects. The gene expression and cytokine profiles of lSSc-PAH patients suggest the presence of activated monocytes, and show markers of vascular injury and inflammation. These genes and factors could serve as biomarkers of PAH involvement in lSSc.
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影响因子：
7.8
作者：
Grigoryev, Dmitry N.;Mathai, Stephen C.;Hassoun, Paul M.
通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1080/17482940802409662
发表时间：
2008-01-01
期刊：
Acute cardiac care
影响因子：
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作者：
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通讯作者：
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