Correlation of theophylline levels in rat exhaled breath and lung tissue after its intravenous injection
Correlation of theophylline levels in rat exhaled breath and lung tissue after its intravenous injection
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静脉注射大鼠呼出气与肺组织中茶碱含量的相关性
DOI：
10.1088/1752-7163/ac6b4b
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发表时间：
2022
影响因子：
3.8
通讯作者：
Kawabe Tsutomu
中科院分区：
文献类型：
--
作者：
Matsushima Miyoko;Tanihata Souma;Kusakabe Junpei;Okahira Momoha;Ito Hiroshi;Yamamoto Atsushi;Yamamoto Masanori;Yamamoto Ryohei;Kawabe Tsutomu
重要的是要知道靶组织中的药物水平，以确定其剂量。一些方法依赖于药物的血液水平来估计其在组织中的浓度，这可能不准确。我们认为呼气气溶胶（EBA）中的药物水平可以更准确地反映肺中试验药物的水平。给大鼠静脉注射支气管扩张剂茶碱，并在给药后10-20 min收集呼出气。呼吸采集后立即采集肺、肝、肾和血液，并使用这些样本检查药代动力学。清醒自由活动的大鼠用于有效地收集来自具有低潮气量的大鼠的呼出气。以呼出水蒸气量估算大鼠呼出气量，呼出气样品中药物浓度以水蒸气量为分母表示。采用泵吸吸附剂的主动进样方法，可准确测定大鼠呼出气中的茶碱。当检查每个样品中茶碱浓度的相关性时，仅在呼出气和肺组织中发现高相关性（r2 = 0.74）。在肺组织药代动力学分析中，认为EBA优于血液。
It is important to know the drug level in the target tissue to determine its dose. Some methods rely on blood levels of a drug to estimate its concentration in the tissues, which can be inaccurate. We thought that drug levels in exhaled breath aerosol (EBA) to give a more accurate value of the level of a test drug in the lung. Rats were intravenously injected with the bronchodilator theophylline and exhaled breath was collected up to 10–20 min after administration. Immediately after breath collection, lung, liver, kidney, and blood were collected and the pharmacokinetics were examined using these samples. Awake free-moving rats were used to efficiently collect exhaled breath from rats with low tidal volume. The amount of exhaled breath of rats was estimated by the amount of exhaled water vapor, and the drug concentration in exhaled breath sample was expressed by the amount of water vapor as the denominator. By using the active sampling method in which the adsorbent is sucked by a pump, theophylline in rat exhaled breath could be measured accurately. When the correlation of theophylline concentration in each sample was examined, a high correlation (r 2= 0.74) was found only in exhaled breath and lung tissue. EBA was considered better than blood in pharmacokinetic analysis of lung tissue.
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使用微透析进行药物分布研究。
DOI：
--
发表时间：
1991
期刊：
Life Science
影响因子：
--
作者：
L. Ståhle;P. Arner;U. Ungerstedt
通讯作者：
U. Ungerstedt
大鼠中茶碱的剂量依赖性消除。
DOI：
10.3109/00498258509045346
发表时间：
1985
期刊：
Xenobiotica; the fate of foreign compounds in biological systems
影响因子：
--
作者：
M. Teunissen;I. Brorens;J. M. Geerlings;D. Breimer
通讯作者：
D. Breimer
在反相 LC-MS/MS 生物分析方法中分析磷脂洗脱以避免基质效应。
影响因子：
1.8
作者：
Steve Silvester;Luke Smith
通讯作者：
Luke Smith
影响因子：
5.7
作者：
Zuurbier, Coert J.;Keijzers, Peter J. M.;Hollmann, Markus W.
通讯作者：
Hollmann, Markus W.
妊娠和非妊娠大鼠茶碱分布的基于生理学的药代动力学模型
DOI：
--
发表时间：
1984
期刊：
Journal of pharmacokinetics and biopharmaceutics
影响因子：
--
作者：
J. Gabrielsson;L. Paalzow;L. Nordström
通讯作者：
L. Nordström