Association between the expression of core 3 synthase and survival outcomes of patients with cholangiocarcinoma.
Association between the expression of core 3 synthase and survival outcomes of patients with cholangiocarcinoma.
复制标题
胆管癌患者的核心3合酶表达与生存结果之间的关联。
DOI：
10.3892/ol.2021.13021
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发表时间：
2021-11
期刊：
影响因子：
2.9
通讯作者：
Narimatsu H
中科院分区：
文献类型：
--
作者：
Boottanun P;Ino Y;Shimada K;Hiraoka N;Angata K;Narimatsu H
胆管癌（CCA）是一种高度侵袭性和转移性的恶性肿瘤，与高死亡率相关，并且难以在早期发现。核心3结构是由β 1，3-N-乙酰氨基葡萄糖转移酶6（core 3 synthase）合成的粘蛋白型O-聚糖，在消化系统尤其是胃肠道杯状细胞中发挥重要作用。据报道，表达核心3蛋白酶的细胞显示出较低的迁移率和侵袭率，以及较低的转移活性。一项免疫组织化学研究还表明，这种酶在结肠的正常上皮细胞中表达，但在结直肠癌细胞中完全消失。本研究旨在确定可用于预测CCA患者预后的生物标志物。用两种抗体G8-144和MECA-79对185个CCA组织的病理标本进行免疫化学染色，这两种抗体分别识别延伸的核心-1 O-聚糖上的核心3合酶和6-硫酸化N-乙酰乳糖胺。统计分析G8-144或MECA-79阳性与患者预后的关系。G8-144阳性表达与远端CCA（dCCA）患者的预后改善相关。G8-144阳性的dCCA患者的死亡率低于阴性表达者。然而，MECA-79的阳性表达与CCA的进展和转移相关，表明其是CCA的不良预后标志物。总之，由于两种抗体均导致镜像染色，因此G8-144和MECA-79参与O-聚糖合成可被认为分别是CCA预后的潜在有利和不利生物标志物。
Cholangiocarcinoma (CCA) is a highly aggressive and metastatic type of malignant carcinoma that is associated with high mortality rates and is difficult to detect at early stages. Core 3 structure is a mucin-type O-glycans synthesized by β1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase), which plays an important role in the digestive system, in particular gastrointestinal goblet cells. It has been reported that core 3 synthase-expressing cells show lower migratory and invasive rates, and lower metastatic activity. A immunohistochemical study also showed that this enzyme was expressed in normal epithelial cells of the colon, but completely disappeared in colorectal cancer cells. The present study aimed to identify biomarkers that could be used to predict the prognosis of patients with CCA. Pathological specimens of 185 CCA tissues were immunohistochemically stained with two antibodies, G8-144 and MECA-79, which recognize core 3 synthase and 6-sulfated N-acetyllactosamine on the extended core-1 O-glycans, respectively. The association between G8-144 or MECA-79 positivity and patient prognosis was statistically analyzed. Positive expression of G8-144 was associated with improved prognosis in patients with distal CCA (dCCA). Patients with dCCA positive for G8-144 showed lower mortality rates than those with negative expression. However, the positive expression of MECA-79 was associated with CCA progression and metastasis, indicating that it is a poor prognostic marker for CCA. In conclusion, as both antibodies resulted in mirror-image staining, the involvement of G8-144 and MECA-79 in O-glycan synthesis could be considered as potential favorable and unfavorable biomarkers, respectively, for CCA prognosis.
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DOI：
10.1002/prca.201300024
发表时间：
2013-10
期刊：
Proteomics. Clinical applications
影响因子：
--
作者：
Ju T;Wang Y;Aryal RP;Lehoux SD;Ding X;Kudelka MR;Cutler C;Zeng J;Wang J;Sun X;Heimburg-Molinaro J;Smith DF;Cummings RD
通讯作者：
Cummings RD
DOI：
10.1146/annurev-pathol-012414-040438
发表时间：
2015
期刊：
Annual review of pathology
影响因子：
--
作者：
Stowell SR;Ju T;Cummings RD
通讯作者：
Cummings RD
影响因子：
3.7
作者：
Doi N;Ino Y;Angata K;Shimada K;Narimatsu H;Hiraoka N
通讯作者：
Hiraoka N
影响因子：
5.7
作者：
Okayama, Hirokazu;Kumamoto, Kensuke;Takenoshita, Seiichi
通讯作者：
Takenoshita, Seiichi
影响因子：
4.7
作者：
Angata, Kiyohiko;Sawaki, Hiromichi;Narimatsu, Hisashi
通讯作者：
Narimatsu, Hisashi