Single Nucleotide Polymorphisms in hsp65 and MACPPE12 Genes of Mycobacterium avium subsp. hominissuis
Single Nucleotide Polymorphisms in hsp65 and MACPPE12 Genes of Mycobacterium avium subsp. hominissuis
复制标题
鸟分枝杆菌亚种 hsp65 和 MACPPE12 基因的单核苷酸多态性。
DOI：
10.1134/s1022795419050120
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发表时间：
2019
影响因子：
0.6
通讯作者：
Narvskaya O. V.
中科院分区：
文献类型：
--
作者：
Starkova D. A.;Iwamoto T.;Vyazovaya A. A.;Molchanov V. M.;Zhuravlev V. Yu.;Vishnevsky B. I.;Narvskaya O. V.
AbstractMycobacterium aviumsubsp.hominissuis(MAH) are typical inhabitants of the environment, which are known as opportunistic pathogens of animals and humans. The aim of our study was to analyze single-nucleotide polymorphisms (SNPs) in thehsp65andMACPPE12genes to characterize the Russian population ofMAHin the context of studying phylogenetic relationships and the evolution of geographically distant populations ofM. aviumsubsp.hominissuis. The sequence analysis of thehsp65andMACPPE12genes was applied for 40MAHstrains isolated from humans (patients with mycobacteriosis). The nucleotide sequences were aligned to the reference genome ofM. aviumsubsp.hominissuis104 (accession no. NC_008595.1). The mutational profiles of Russian strains were compared with those isolated in other countries. In total, 40MAHstrains were classified into three differenthsp65sequevars: code 1, code 2, and code 3. The majority ofMAHstrains (72.5%) belonged to code 1, the same sequevar as forMAHstrain 104. The sequence analysis of theMACPPE12gene revealed 20 SNPs grouped into nine sequevars at the nucleic acid level: NA01, NA02, NA03, NA06, NA10, NA13, NA14, NA19, and NA_Rus01. Eight out of 20 SNPs were nonsynonymous, resulting in seven sequevars at the amino acid level: AA01, AA02, AA04, AA07, AA08, AA13, and AA_Rus01. The sequevar AA02 consisted of three different NA variants with synonymous SNPs profiles: NA02, NA03, and NA06. Half of theMAHstrains belonged to the sequevar AA02 (type NA02). The predominant cluster AA02 (type NA02)/code 1 and the unique variant AA_Rus01 (NA_Rus01) were identified amongMAHstrains from Russia. Thus, we confirmed the relative conservativeness of the nucleotide sequence of thehsp65gene but the polymorphism of theMACPPE12gene. At the same time, a comparative analysis of the SNPs profiles of thehsp65andMACPPE12genes allowed us to identify differences and similarities between geographically distant populations ofMAH, which highlighted the variability of the global population ofM. aviumspecies.
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影响因子：
3.7
作者：
Kim SY;Jeong BH;Park HY;Jeon K;Han SJ;Shin SJ;Koh WJ
通讯作者：
Koh WJ
影响因子：
9.4
作者：
Turenne, CY;Semret, M;Behr, MA
通讯作者：
Behr, MA
影响因子：
0.6
作者：
D. Starkova;T. Otten;I. Mokrousov;A. Vyazovaya;B. Vishnevsky;O. Narvskaya
通讯作者：
O. Narvskaya
影响因子：
2.2
作者：
Bartos, M;Hlozek, P;Pavlik, I
通讯作者：
Pavlik, I
影响因子：
3.2
作者：
Scherrer S;Landolt P;Carroli N;Stephan R
通讯作者：
Stephan R