The transcription factor interferon regulatory factor-1 is expressed during both early G1 and the G1/S transition in the prolactin-induced lymphocyte cell cycle.
The transcription factor interferon regulatory factor-1 is expressed during both early G1 and the G1/S transition in the prolactin-induced lymphocyte cell cycle.
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转录因子干扰素调节因子-1 在催乳素诱导的淋巴细胞细胞周期的早期 G1 和 G1/S 转变期间表达。
作者：
Anne M. Stevens;L. Yu
PRL诱导静止的Nb2大鼠t淋巴瘤细胞进行有丝分裂。在PRL刺激下，转录因子干扰素调节因子-1 （IRF-1）在Nb2细胞中被诱导为一种新的t细胞激活基因。令人惊讶的是，IRF-1在单个prl诱导的生长周期中表达两次：第一次在G1期早期，在15分钟至2小时的瞬时峰值中表达，第二次在G1/S期转变期间，在8小时开始出现一个更宽的峰值。IRF-1 mRNA的不寻常的双相表达两次都伴随着从头开始的IRF-1蛋白合成。然而，IRF-1蛋白的周转率在G1期和S期似乎是不同的。IRF-1蛋白在G1期表达的半衰期约为25 min，而在S期表达的半衰期约为60 min。通过在G1期间的不同时间洗涤PRL，我们发现PRL暴露的长度、IRF-1 mRNA表达的第二峰与DNA合成之间存在直接相关性。我们的数据表明，PRL和一种假定的核介质IRF-1可能在细胞周期的两个不同阶段起重要作用：首先在细胞周期激活中，然后在S期进展中。
PRL induces quiescent Nb2 rat T-lymphoma cells to undergo mitogenesis. Upon PRL stimulation, the transcription factor interferon regulatory factor-1 (IRF-1) is induced as a novel T-cell activation gene in Nb2 cells. Surprisingly, IRF-1 is expressed twice during a single PRL-induced growth cycle: first during the early G1 phase, in an immediate transient peak from 15 min to 2 h, and second during the G1/S phase transition, in a broader peak beginning at 8 h. The unusual biphasic expression of IRF-1 mRNA is accompanied both times by de novo IRF-1 protein synthesis. However, the rate of IRF-1 protein turnover appears to be different in G1 and S phases. IRF-1 protein expressed in G1 exhibits a half-life of about 25 min, whereas in the S phase, the half-life is about 60 min. By washing out PRL at various times during G1, we found a direct correlation among the length of PRL exposure, the second peak of IRF-1 mRNA expression, and DNA synthesis. Our data suggest that PRL and one putative nuclear mediator, IRF-1, may be important in two distinct phases of the cell cycle: first in cell cycle activation, and then in S phase progression.
克隆 T 淋巴细胞中的原癌基因表达：丝裂原和生长因子诱导不同的表达模式。
影响因子：
8
作者：
Reed,JC;Alpers,JD;Scherle,PA;Hoover,RG;Nowell,PC;Prystowsky,MB
通讯作者：
Prystowsky,MB
由促有丝分裂和非促有丝分裂 T3 和 T11 激活信号触发的 T 细胞中核原癌基因的差异表达。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Shipp,MA;Reinherz,EL
通讯作者：
Reinherz,EL
新生儿给予催乳素抗血清会改变 BALB/c 雌性小鼠胸腺和脾脏中 T 淋巴细胞和 B 淋巴细胞的发育模式。
DOI：
10.1073/pnas.85.19.7404
发表时间：
1988
影响因子：
11.1
作者：
Russell,DH;Mills,KT;Talamantes,FJ;Bern,HA
通讯作者：
Bern,HA