The pathogenesis of optic neuritis caused by Angiostrongylus cantonensis in BALB/c mice.
The pathogenesis of optic neuritis caused by Angiostrongylus cantonensis in BALB/c mice.
复制标题
广州管圆线虫引起的BALB/c小鼠视神经炎的发病机制
DOI：
10.1186/1756-3305-7-339
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发表时间：
2014-07-22
影响因子：
3.2
通讯作者：
Wu ZD
中科院分区：
文献类型：
--
作者：
Feng Y;Zeng X;Li WH;Wang WC;Chen W;Ou-Yang LS;Sun X;Feng F;Wu ZD
在东南亚，最常见的脑膜炎病因之一是管圆线虫病或广州管圆线虫感染。虽然这种线虫通常居住在大鼠的肺动脉中，但它在其他宿主（如人类）中的偶然发生可引起视神经炎并导致严重的视力后遗症。然而，目前在这方面还没有进行系统的研究。为了研究视神经炎的发病机制，以小鼠为新动物模型，分别在第7d、第14d和第21d对广东棘球绦虫进行研究和攻毒。分别于第7d、14d、21d检查视网膜电图（ERG）、视觉诱发电位（VEP）、眼镜检查及组织学检查，并于第14d应用三苯二胺（TBD）治疗视神经炎1周，观察其治疗效果。第14d和21d广东棘球蚴感染引起小鼠视网膜和视神经外膜明显的炎症细胞浸润，第21d出现视神经纤维脱髓鞘和视网膜神经节肿胀。第21天VEP潜伏期延长，ERG振幅下降。经TBD治疗后，感染小鼠的视网膜和视神经炎症得到缓解，但在第21天和第28天VEP潜伏期和ERG振幅没有改善。本研究在小鼠模型中提供了广东棘球蚴可引起视神经炎、视神经脱髓鞘和视网膜神经节细胞损伤的证据。TBD单独治疗可以改善视神经炎的症状，但对视力恢复没有帮助，提示应同时使用神经保护剂和地塞米松，同时治疗感染，以保护视神经和神经节细胞。此外，由于广州棘球绦虫引起的小鼠视神经炎的症状与多发性硬化症（MS）患者的视神经炎相似，我们认为本研究提供的BALB/c小鼠模型可能有助于探索MS患者视神经炎的治疗方法。
One of the most common causes of meningitis in South East Asia is angiostrongyliasis or infection by the parasitic nematode Angiostrongyliasis cantonensis. Although this nematode usually resides in the pulmonary arteries of rats, its incidental occurence in other hosts such as humans can cause optic neuritis and lead to serious vision sequelae. Nevertheless, there are currently no systematic studies conducted in this area. In order to study the pathogenesis of optic neuritis, mice were tried as a new animal model to study and challenge with A. cantonensis on 7d, 14d and 21d, respectively. Electroretinogram (ERG), visual evoked potential (VEP), ophthalmoscopy and histology were examined on day 7d, 14d and 21d and tribendimidine (TBD) was later used to treat optic neuritis on day 14d for a week to evaluate its therapeutic effects. Infection of A. cantonensis caused obvious inflammatory cell infiltration in the retina and optic nerve adventitia in day 14d and 21d followed by optic nerve fiber demyelination and retinal ganglion swelling at day 21d in the challenged mice. Prolonged VEP latency and decreased ERG amplitude were also observed on day 21. After treatment of TBD in the infected mice, retinal and optic nerve inflammation were alleviated, but VEP latency and ERG amplitude did not improve on day 21d and 28d. The current study provides evidence that A. cantonensis can cause optic neuritis along with optic nerve demyelination and retinal ganglion cell damage in a mouse model. TBD alone treatment can improve the symptoms of optic neuritis, but does not aid in vision recovery, suggesting that both neuroprotective agents and Dexamethasone should be administered, along with treatment for the infection, to protect the optic nerve and ganglion cells. Furthermore, as the symptoms of optic neuritis caused by A. cantonensis in mice are similar to the optic neuritis in multiple sclerosis (MS) human patients, we suggest that the BALB/c mouse model provided in this study may be useful to explore therapies of optic neuritis in MS patients.
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影响因子：
3.4
作者：
Shindler, Kenneth S.;Ventura, Elvira;Rostami, Abdolmohamad
通讯作者：
Rostami, Abdolmohamad
影响因子：
158.5
作者：
BECK, RW;CLEARY, PA;ATWELL, CW
通讯作者：
ATWELL, CW
影响因子：
4.2
作者：
Liu, IH;Chung, YM;Cho, WL
通讯作者：
Cho, WL
影响因子：
3.4
作者：
Quinn TA;Dutt M;Shindler KS
通讯作者：
Shindler KS
影响因子：
5.8
作者：
Blecharz, Kinga G.;Haghikia, Aiden;Foerster, Carola Y.
通讯作者：
Foerster, Carola Y.