Beta-Amylase and Phosphatidic Acid Involved in Recalcitrant Seed Germination of Chinese Chestnut.
Beta-Amylase and Phosphatidic Acid Involved in Recalcitrant Seed Germination of Chinese Chestnut.
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β-淀粉酶和磷脂酸参与板栗顽拗种子萌发
DOI：
10.3389/fpls.2022.828270
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发表时间：
2022
影响因子：
5.6
通讯作者：
Xing Y
中科院分区：
文献类型：
--
作者：
Liu Y;Zhang Y;Zheng Y;Nie X;Wang Y;Yu W;Su S;Cao Q;Qin L;Xing Y
板栗（Castanea mollissima）是一种种子顽固的植物，是坚果和森林生态系统服务的重要来源。在自然生境中，顽固性种子的发芽率低，在干燥和低温条件下发芽率降低。栽培板栗种子的发芽率显著高于野生板栗种子。为探究栽培板栗种子发芽率较高的原因，通过基因组比对，检测出野生板栗与栽培板栗基因组间的113,524个结构变异。对60份板栗材料的sv进行基因分型，发现板栗驯化过程中等位基因频率发生了变化，部分sv是控制种子萌发的重叠基因。转录组分析显示，在培养种子的强选择基因中，脱落酸合成基因下调，β -淀粉酶合成基因上调。另一方面，激素和酶活性分析表明，栽培种子内源ABA水平降低，β -淀粉酶活性升高。这些结果揭示了栽培种子发芽率较高的原因。此外，磷脂酸合成基因在野生板栗种子萌发阶段高度表达，可能在种子萌发的顽固性中起作用。这些发现为研究野生种子萌发的调控和促进森林生态系统的自然更新和演替提供了新的思路。
Chinese chestnut (Castanea mollissima), a species with recalcitrant seeds, is an important source of nuts and forest ecosystem services. The germination rate of recalcitrant seeds is low in natural habitats and decreases under conditions of desiccation and low temperature. The germination rate of cultivated Chinese chestnut seeds is significantly higher than that of wild seeds. To explore the reasons for the higher germination rate of cultivated seeds in Chinese chestnut, 113,524 structural variants (SVs) between the wild and cultivated Chinese chestnut genomes were detected through genome comparison. Genotyping these SVs in 60 Chinese chestnut accessions identified allele frequency changes during Chinese chestnut domestication, and some SVs are overlapping genes for controlling seed germination. Transcriptome analysis revealed downregulation of the abscisic acid synthesis genes and upregulation of the beta-amylase synthesis genes in strongly selected genes of cultivated seeds. On the other hand, hormone and enzyme activity assays indicated a decrease in endogenous ABA level and an increase in beta-amylase activity in cultivated seeds. These results shed light on the higher germination rate of cultivated seeds. Moreover, phosphatidic acid synthesis genes are highly expressed in seed germination stages of wild Chinese chestnut and may play a role in recalcitrant seed germination. These findings provide new insight into the regulation of wild seed germination and promote natural regeneration and succession in forest ecosystems.
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影响因子：
7
作者：
Chen, Hua;Patterson, Nick;Reich, David
通讯作者：
Reich, David
影响因子：
12.3
作者：
Haas, Brian J.;Salzberg, Steven L.;Zhu, Wei;Pertea, Mihaela;Allen, Jonathan E.;Orvis, Joshua;White, Owen;Buell, C. Robin;Wortman, Jennifer R.
通讯作者：
Wortman, Jennifer R.
影响因子：
7.2
作者：
Katagiri, T;Ishiyama, K;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
影响因子：
11.6
作者：
Kim, Dong Hwan;Yamaguchi, Shinjiro;Choi, Giltsu
通讯作者：
Choi, Giltsu