Effect of experimental neutropenia on initial gingivitis in dogs.
Effect of experimental neutropenia on initial gingivitis in dogs.
复制标题
实验性中性粒细胞减少症对狗初始牙龈炎的影响。
DOI：
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
H. Schroeder
中科院分区：
文献类型：
--
作者：
R. Attström;H. Schroeder
通过在比格犬的初始牙龈炎期间诱导实验性中性粒细胞减少来研究嗜中性粒细胞在牙龈胶原损失中的作用。通过反复注射兔抗中性粒细胞血清，在三只牙龈正常的动物中诱导中性粒细胞减少4 d。在中性粒细胞减少症期间，允许在牙齿上形成微生物菌斑。在第0天和第4天采集交界（龈沟）白细胞和牙龈液样本。在第4天获得颊牙龈的块活检。染色的半切片和半切片用于组织计量学和体视学组织分析。从第0天到第4天，所有犬的牙龈液流量增加，而仅一只犬的交界处白细胞增加。龈下菌斑在大多数活检中已经形成，在交界上皮中很少有嗜中性粒细胞存在。在结合上皮淋巴细胞下的冠状结缔组织中，弥漫性散在结构异常的嗜中性粒细胞和单核细胞/巨噬细胞。牙龈胶原主要被炎性细胞置换而非溶解。这些数据表明，嗜中性粒细胞可能有助于牙龈胶原蛋白的损失在最初的牙龈炎在狗。嗜中性粒细胞似乎也是重要的限制龈下菌斑生长沿着牙齿表面。
The role of neutrophilic granulocytes in the loss of gingival collagen has been studied by inducing experimental neutropenia during initial gingivitis in beagle dogs. Neutropenia was induced for 4 d in three animals with normal gingiva by repeated injections of rabbit anti-neutrophil serum. During neutropenia microbial plaque was allowed to form on the teeth. Samples of junctional (crevicular) leukocytes and gingival fluid were taken on days 0 and 4. Block biopsies of buccal gingiva were obtained on day 4. Stained semi- and ultrathin sections were used for histometric and stereologic tissue analysis. Gingival fluid flow increased from day 0 to day 4 in all dogs while junctional leukocytes increased in one dog only. Subgingival plaque had formed in most biopsies, and in the junctional epithelium very few neutrophilic granulocytes were present. In the coronal connective tissue subjacent to the junctional epithelium lymphoid cells, structurally abnormal neutrophilic granulocytes and monocytes/macrophages were diffusely scattered. The gingival collagen appeared mainly displaced by the inflammatory cells rather than dissolved. The data suggest that neutrophilic granulocytes may contribute to the loss of gingival collagen during initial gingivitis in dogs. The neutrophils also seem to be of importance for the limitation of subgingival plaque growth along the tooth surface.
影响因子：
3.5
作者：
Passo,SA;Tsai,CC;McArthur,WP;Leifer,C;Taichman,NS
通讯作者：
Taichman,NS